Syringaldehyde Attenuates Chronic Constriction Injury-Induced Neuropathic Pain In Sprague-Dawley Rats Via Pge2-Inos Inhibition
Keywords:
CCI, Syringaldehyde, PGE2, iNOS, Allodynia, Neuropathic pain
Abstract
Neuropathic pain is a one of the most unbearable pain condition severely affecting day-to-day life in affected patients. Syringaldehyde is a compound isolated from various plant species. Syringaldehyde has shown several pharmacological properties including anti-inflammatory, anti-oxidant activities. In this work, to assess in-vivo pharmacological effect of Syringaldehyde in development and maintainace of neuropathic pain, male SD rats were subjected to CCI ligation surgery of sciatic nerve with assessment of mechanical allodynia using von Frey filaments and cold allodynia using acetone test from post-surgery day 3 onwards. CCI rats were given Syringaldehyde orally from day 7 to 14 and pain responses were recorded on day 7, 14. At the end of study, expression levels of PGE2 and iNOS mRNA was assessed in sciatic nerve of CCI rats. CCI surgery in rats developed mechanical and cold allodynia from post-surgery day 3 onwards with peak allodynia observed at day 7, and allodynia was maintained till day 14. Treatment with Syringaldehyde for from day 7 to day 14 showed reversal of mechanical and cold allodynia inhibition of CCI rats when allodynia was assessed on day 7 and day 14. Moreover, treatment with Syringaldehyde decreased mRNA expression levels of PGE2 and iNOS in sciatic nerve. The results from this study demonstrated the analgesic potential of Syringaldehyde in neuropathic pain induced by CCI surgery which may be mediated via inhibition of PGE2 and iNOS.
References
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26. Singh H., Bhushan S., Arora R., Singh Buttar H., Arora S., Singh B. (2017). Alternative Treatment Strategies for Neuropathic Pain: Role of Indian Medicinal Plants and Compounds of Plant Origin-A Review. Biomed. Pharmacother. 92, 634–650. Doi-10.1016/j.biopha.2017.05.079
27. Sobeh M, Mahmoud MF, Rezq S, Abdelfattah MAO, Mostafa I, Alsemeh AE, El-Shazly AM, Yasri A, Wink M. Haematoxylon campechianum Extract Ameliorates Neuropathic Pain via Inhibition of NF- KB/TNF-a/NOX/INOS Signalling Pathway in a Rat Model of Chronic Constriction Injury. Biomolecules. 2020; 10(3):386. https://doi.org/10.3390/biom10030386
28. Sobeh M, Mahmoud MF, Rezq S, Alsemeh AE, Sabry OM, Mostafa I, Abdelfattah MAO, Ait El-Allem K, El-Shazly AM, Yasri A, et al. Salix tetrasperma Roxb. Extract Alleviates Neuropathic Pain in Rats via Modulation of the NF-kB/TNF-a/NOX/iNOS Pathway. Antioxidants. 2019; 8(10):482. https://doi.org/10.3390/antiox8100482
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31. Xu B, Descalzi G, Ye H-R, Zhuo M, Wang Y-W. Translational Investigation and Treatment of Neuropathic Pain. Molecular Pain. 2012;8. doi:10.1186/1744-8069-8-15
32. Zanjani TM, Sabetkasaei M, Mosaffa N, Manaheji H, Labibi F, Farokhi B. Suppression of interleukin-6 by minocycline in a rat model of neuropathic pain. Eur J Pharmacol. 2006 May 24;538(1-3):66-72. doi: 10.1016/j.ejphar.2006.03.063.
2. Baron R. Mechanisms of disease: neuropathic pain--a clinical perspective. Nat Clin Pract Neurol. 2006 Feb;2(2):95-106. doi: 10.1038/ncpneuro0113.
3. Basbaum AI, Fields HL. Endogenous pain control systems: brainstem spinal pathways and endorphin circuitry. Annu Rev Neurosci. 1984;7:309-38. doi: 10.1146/annurev.ne.07.0301 b84.001521.
4. Bennett GJ, Xie YK. A peripheral mononeuropathy in rat that produces disorders of pain sensation like those seen in man. Pain. 1988 Apr;33(1):87-107. doi: 10.1016/0304-3959(88)90209-6.
5. Bennett, G. J., and Xie, Y.-K. (1988). A peripheral mononeuropathy in rat that produces disorders of pain sensation like those seen in man. Pain 33, 87–107. doi: 10.1016/0304-3959(88)90209-6
6. Bernetti A., Agostini F., de Sire A., Mangone M., Tognolo L., Di Cesare A., et al. (2021). Neuropathic Pain and Rehabilitation: A Systematic Review of International Guidelines. Diagnostics 11 (1), 74. Doi-10.3390/diagnostics11010074
7. Bozkurt AA, Mustafa G, Tarık A, Adile O, Murat SH, Mesut K, Yıldıray K, Coskun S, Murat C. Syringaldehyde exerts neuroprotective effect on cerebral ischemia injury in rats through anti-oxidative and anti-apoptotic properties. Neural Regen Res. 2014 Nov 1;9(21):1884-90. doi: 10.4103/1673-5374.145353.
8. Chaplan S. R., Bach F. W., Pogrel J. W., Chung J. M., Yaksh T. L. (1994). Quantitative assessment of tactile allodynia in the rat paw. J. Neurosci. Methods 53, 55–63. 10.1016/0165-0270(94)90144-9
9. Choi Y, Yoon YW, Na HS, Kim SH, Chung JM (1994). Behavioral signs of ongoing pain and cold allodynia in a rat model of neuropathic pain. Pain, 59 (3): 369-376. DOI: 10.1016/0304-3959(94)90023-X
10. De Jongh RF, Vissers KC, Meert TF, Booij LHDJ, De Deyne CS, Heylen RJ. The role of interleukin-6 in nociception and pain. Anesth Analg. 2003 Apr;96(4):1096-1103. doi: 10.1213/01.
11. Finnerup NB, Sindrup SH, Jensen TS. The evidence for pharmacological treatment of neuropathic pain. Pain. 2010 Sep;150(3):573-581. doi: 10.1016/j.pain.2010.06.019.
12. Gao Y, Jiang W, Dong C, Li C, Fu X, Min L, Tian J, Jin H, Shen J. Anti-inflammatory effects of sophocarpine in LPS-induced RAW 264.7 cells via NF-kB and MAPKs signaling pathways. Toxicol In Vitro. 2012 Feb;26(1):1-6. doi: 10.1016/j.tiv.2011.09.019.
13. Jaggi AS, Singh N. Differential effect of spironolactone in chronic constriction injury and vincristine-induced neuropathic pain in rats. Eur J Pharmacol. 2010 Dec 1;648(1-3):102-9. doi: 10.1016/j.ejphar.2010.08.050.
14. Jin Y, Sato J, Yamazaki M, Omura S, Funakubo M, Senoo S, Aoyama M, Mizumura K. Changes in cardiovascular parameters and plasma norepinephrine level in rats after chronic constriction injury on the sciatic nerve. Pain. 2008 Apr;135(3):221-231. doi: 10.1016/j.pain.2007.05.020.
15. Kaur, G., Bedi, O., Sharma, N., Singh, S., Deshmukh, R. and Kumar, P. (2016) Anti-hyperalgesic and anti-nociceptive potentials of standardized grape seed proanthocyanidin extract against CCI-induced neuropathic pain in rats. Journal of Basic and Clinical Physiology and Pharmacology, Vol. 27 (Issue 1), pp. 9-17. https://doi.org/10.1515/jbcpp-2015-0026
16. Korhonen R, Lahti A, Kankaanranta H, Moilanen E. Nitric oxide production and signaling in inflammation. Curr Drug Targets Inflamm Allergy. 2005 Aug;4(4):471-9. doi: 10.2174/1568010054526359.
17. Kumar A, Agarwal K, Maurya AK, Shanker K, Bushra U, Tandon S, Bawankule DU. Pharmacological and phytochemical evaluation of Ocimum sanctum root extracts for its antiinflammatory, analgesic and antipyretic activities. Pharmacogn Mag. 2015 May;11(Suppl 1):S217-24. doi: 10.4103/0973-1296.157743.
18. Ma, W. and Quirion, R. (2005), Up-regulation of interleukin-6 induced by prostaglandin E2 from invading macrophages following nerve injury: an in vivo and in vitro study. Journal of Neurochemistry, 93: 664-673. https://doi.org/10.1111/j.1471-4159.2005.03050.x
19. Mahmoud MF, Rezq S, Alsemeh AE, Abdelfattah MAO, El-Shazly AM, Daoud R, El Raey MA and Sobeh M (2021) Potamogeton perfoliatus L. Extract Attenuates Neuroinflammation and Neuropathic Pain in Sciatic Nerve Chronic Constriction Injury-Induced Peripheral Neuropathy in Rats. Front. Pharmacol. 12:799444. doi: 10.3389/fphar.2021.799444
20. Mahmoud MF, Rezq S, Alsemeh AE, Abdelfattah MAO, El-Shazly AM, Daoud R, El Raey MA and Sobeh M (2021) Potamogeton perfoliatus L. Extract Attenuates Neuroinflammation and Neuropathic Pain in Sciatic Nerve Chronic Constriction Injury-Induced Peripheral Neuropathy in Rats. Front. Pharmacol. 12:799444. doi: 10.3389/fphar.2021.799444
21. Meller ST, Pechman PS, Gebhart GF, Maves TJ. Nitric oxide mediates the thermal hyperalgesia produced in a model of neuropathic pain in the rat. Neuroscience. 1992 Sep;50(1):7-10. doi: 10.1016/0306-4522(92)90377-e.
22. Pradhan AA, Yu XH, Laird JM. Modality of hyperalgesia tested, not type of nerve damage, predicts pharmacological sensitivity in rat models of neuropathic pain. Eur J Pain. 2010 May;14(5):503-9. doi: 10.1016/j.ejpain.2009.08.010.
23. Rezq, S., Alsemeh, A.E., D'Elia, L. et al. Thymus algeriensis and Thymus fontanesii exert neuroprotective effect against chronic constriction injury-induced neuropathic pain in rats. Sci Rep 10, 20559 (2020). https://doi.org/10.1038/s41598-020-77424-0
24. Rutten K, Gould SA, Bryden L, Doods H, Christoph T, Pekcec A. Standard analgesics reverse burrowing deficits in a rat CCI model of neuropathic pain, but not in models of type 1 and type 2 diabetes-induced neuropathic pain. Behav Brain Res. 2018 Sep 17;350:129-138. doi: 10.1016/j.bbr.2018.04.049.
25. Saliba S. W., Jauch H., Gargouri B., Keil A., Hurrle T., Volz N., Mohr F., Stelt M., Bräse S., Fiebich B. L. (2018): Anti-neuroinflammatory effects of GPR55 antagonists in LPS-activated primary microglial cells. J. Neuroinflammation. 15, 322.
26. Singh H., Bhushan S., Arora R., Singh Buttar H., Arora S., Singh B. (2017). Alternative Treatment Strategies for Neuropathic Pain: Role of Indian Medicinal Plants and Compounds of Plant Origin-A Review. Biomed. Pharmacother. 92, 634–650. Doi-10.1016/j.biopha.2017.05.079
27. Sobeh M, Mahmoud MF, Rezq S, Abdelfattah MAO, Mostafa I, Alsemeh AE, El-Shazly AM, Yasri A, Wink M. Haematoxylon campechianum Extract Ameliorates Neuropathic Pain via Inhibition of NF- KB/TNF-a/NOX/INOS Signalling Pathway in a Rat Model of Chronic Constriction Injury. Biomolecules. 2020; 10(3):386. https://doi.org/10.3390/biom10030386
28. Sobeh M, Mahmoud MF, Rezq S, Alsemeh AE, Sabry OM, Mostafa I, Abdelfattah MAO, Ait El-Allem K, El-Shazly AM, Yasri A, et al. Salix tetrasperma Roxb. Extract Alleviates Neuropathic Pain in Rats via Modulation of the NF-kB/TNF-a/NOX/iNOS Pathway. Antioxidants. 2019; 8(10):482. https://doi.org/10.3390/antiox8100482
29. Stanikunaite R, Khan SI, Trappe JM, Ross SA. Cyclooxygenase-2 inhibitory and antioxidant compounds from the truffle Elaphomyces granulatus. Phytother Res. 2009 Apr;23(4):575-8. doi: 10.1002/ptr.2698.
30. Wu J, Fu YS, Lin K, Huang X, Chen YJ, Lai D, Kang N, Huang L, Weng CF. A narrative review: The pharmaceutical evolution of phenolic syringaldehyde. Biomed Pharmacother. 2022 Sep;153:113339. doi: 10.1016/j.biopha.2022.113339.
31. Xu B, Descalzi G, Ye H-R, Zhuo M, Wang Y-W. Translational Investigation and Treatment of Neuropathic Pain. Molecular Pain. 2012;8. doi:10.1186/1744-8069-8-15
32. Zanjani TM, Sabetkasaei M, Mosaffa N, Manaheji H, Labibi F, Farokhi B. Suppression of interleukin-6 by minocycline in a rat model of neuropathic pain. Eur J Pharmacol. 2006 May 24;538(1-3):66-72. doi: 10.1016/j.ejphar.2006.03.063.
Published
2024-09-09
How to Cite
Annasaheb S. Kalange, Murugan Vedigounder, & Geetha K Mukundan. (2024). Syringaldehyde Attenuates Chronic Constriction Injury-Induced Neuropathic Pain In Sprague-Dawley Rats Via Pge2-Inos Inhibition. Revista Electronica De Veterinaria, 25(1), 1158 -1164. https://doi.org/10.69980/redvet.v25i1.835
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