Determination of Lethal concentration (LC50) of copper sulphate in Labeo Rohita.

  • Rangampeta Rajeshwari
  • Dr. S. Padmaja
Keywords: LC50, Toxicity, CuSo4. 5H2o, Labeo rohita

Abstract

The Present study determination of (LC50 Values) acute toxicity of Indian major cart Labeo Rohita obtain the test organisms 160 Fish fingerlings from the Lake irrespective of sex with the help of local Fisherman, the fish divided into four groups to maintain the aeration all the daily observed and dead fishes removed immediately fishes were rinsed with 0.1% and 4k Mn o4 to avoid infection and were acclimatized to two weeks for laboratory prior to experiments 8 experimental groups (with 3 replicates) and experimental dose of copper sulphate 20mg/L, 40mg/L, 60 mg/L, 80mg/L, 100mg/L, 120mg/L, 140mg/L, 280mg/L along with control (0 mg/L). Physicochemical parameters such as pH (7.2), Turbidity, Biological Oxygen demand (BOD)(3.17), Chemical Oxygen Demand (COD)(3.47), Hardness(240.75), Alkalinity(80.25). The LC50 value at 96 hr was found to be 52.04 mg/L to Labeo rohita. Copper concentration was more toxic to Labeo rohita.  

Author Biographies

Rangampeta Rajeshwari

Research Scholar Dept. of Zoology, University College of Science, Osmania University-Hyderabad.

Dr. S. Padmaja

Associate Professor Dept. of Zoology, University College of Science, Osmania University-Hyderabad.

References

Ramakrishna, R, Shipton, T.A.,Hasan, M.R (2013). Feeding and feed management of Indian major carp in Andhra Pradesh, Indian. FAO Fisheries and aquaculture Technical Paper No.578.Rome, FAO.pp90.

Dahanukar, N. (2010). "Labeo rohita". IUCN Red List of Threatened Species. 2010: e.T166619A6248771.

3.Froese, Rainer and Pauly, Daniel, eds. (2013). "Labeorohita" in FishBase. May 2013 version

Khangarot BS. Histopathological changes in the branchial apparatus of Punctius sopore (Ham.) subjected to toxic doses of Zinc. Arch. Hydrobiol, 1982; 93:352-358

Nilkant GV, Sawant KB. Studies on accumulation and Histopathology of gills after exposure to sublethal concentration of Hexavalent chromium and effect on the oxygen consumption in Scylla serrata (Forskal). Poll. Res, 1993; 12(1):11-18.

Alderdice DF. The detection and measurement of water pollution biological assys, J Fish Res Bd Can. 1966; 25:1- 11.

Nagratnamma R, Rammurthi R, Comparitive evaluation of methyl parathion toxicity to some selected freshwater organisms. Curr. Sci, 1981; 50:334-335

Callow P. General principles and overview. In: P. Callow editors. Handbook of Ecotoxicology. Blackwell Scientific Publ, 1993

Rand GM, Wells PG, McCarty LS. Introduction to aquatic toxicology. In: Fundamental of Aquatic Toxicology: Effects, Environmental fate and Risk assessment; Taylor& Francis, 1995, 3-67.

Das S, Sahu B. Interaction of pH with mercuric chloride toxicity to penaeid prawns from a tropical estuary, East Coast of India: enhanced toxicity at low pH. Chemosphere, 2005; 58(9):1241-8

Witeska M, Jezierska B. The effect of environmental factors on metal toxicity of fish. Fresen. Environ. Bull, 2003; 12:824-9

Ebrahimpour M, Alipour H, Rakhshah S. Influence of water hardness on acute toxicity of copper and zinc on fish. Toxicol. Ind. Health. 2010; 26:361-365.

Straus DL. The acute toxicity of copper to blue Tilapia in dilutions of settled pond water. Aquaculture, 2003; 219:233-240

P Pandari Reddy, Department of Zoology, Osmania University, Hyderabad-500007, India.

R Jgadeshwarulu, , Department of Zoology, Osmania University, Hyderabad-500007, India.

G Sunitha Devi, , Department of Zoology, Osmania University, Hyderabad-500007, India.

Mohammad, Mahamood, Mehjbeen Javed, Saleh, S. Alhewairni, Farhana Zahir, Ashok Kumar Sah, and Md. Irshad Ahmad, Labeo rohita, a bioindicator for water quality and associated biomarkers of heavy metal toxicity in the year 2021.

Javed M, Usmani N et al., (2017) multiple biomarker responses (serum biochemistry, oxidative stress genotoxicity and histopsthology) in Channa Panctatus exposed to heavy metal loaded waste water.

Sabullah MK et al., Heavy metal biomarker: fish behaviour, cellular alteration enzymatic reaction and proteomics approaches (2015).

Abah et al., (2016), preliminary assessment of some heavy ,metals pollution status of Lisikili river water in Zambezi region , Namebia.

Shah N et al.,(2020), monitoring bioaccumulation I in gills and muscle tissues) hematology and genotoxic alteration in Ctenopharyngodon idlna exposed to selected heavy metals BioMedres Intem

Luis et al., 2019, trends in aquaculture sciences from now to use Nanotechnology for disease control. (https//doi.org/10.1111/rag.12229).

Waiwood et al., the effect of copper hardness and pH on the growth of rainbow trout sabno gaindneri.

Afaghi et al.,2020 effects of exposure to sub-lethal concentrations of copper on hematologiacal and hystopathological alterations in common corp cyprinus carpio (https.//doi.org/10.22037/aab)

Published
2024-08-16
How to Cite
Rangampeta Rajeshwari, & Dr. S. Padmaja. (2024). Determination of Lethal concentration (LC50) of copper sulphate in Labeo Rohita. Revista Electronica De Veterinaria, 25(1), 761-766. https://doi.org/10.69980/redvet.v25i1.701
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Articles