Prevalence of Beta-Lactamase Producing Genes in Escherichia Coli Isolated from Diabetic Foot Ulcers: A Hospital Based Study from South-Western Maharashtra

  • Dipak S. , Kailas D. Datkhile, Geeta S. Karande, Kalpana D. Kale, Satish R. Patil
Keywords: Diabetic foot ulcer, E.coli, ESBL, MBL, NDM, TEM, VIM


The antibiotic resistance in the bacteria inhabiting diabetic foot ulcers (DFU) is a major hurdle during medical treatment. Extended-spectrum beta-lactamase (ESBL) and Metallo-beta-lactamase (MBL) production in bacteria is one of the most prevalent beta lactam antibiotic resistance mechanisms. Resent literature suggested that E. coli is one of most abundant Gram negative bacteria found in DFUs. We studied the antibiotic resistance and the prevalence of the genes involved in beta lactamase production in theE. coli isolated from the DFU patients in Maharashtra, India. We isolated a total of 91 E. coli isolates from the diabetic patients and subjected to varies biochemical tests including ESBL and MBL tests. Antibiotic resistance in all the isolates was tested using Ampicillin, Amoxicillin, Piperacillin, Cefuroxime, Ceftriaxone, Cefoperazone, Cefepime, Imipenem, Meropenem, Amikacin, Gentamicine, and Ciprofloxacin. The presence of TEMESBL, SHVESBL, CTX-MESBL,NDM-1bla, KPCbla, OXA-48bla, and VIMbla genes was evaluated by using specific primers and PCR. Among isolates 56.04% were positive for ESBL production and 48.35% were positive for MBL production. All the isolates showed varying degree of resistance towards antibiotics used. More than 50% isolates were vulnerable to carbapenem and aminoglycoside antibiotics. Genetic analysis revealed that NDM-1bla is the most prevalent gene is the E. coli isolates followed by TEMESBL, CTX-MESBL, and KPCbla.VIMbla is the least abundant gene found in the E. coli isolates. The results suggest that all E. coli isolates were multi-drug resistant. There was no association between the presence of a particular gene and antibiotic resistance in the isolates. Further studies considering all bacterial isolates from DFUs for antibacterial resistance and genetic characterization are necessary to understand the genetic basis of resistance.


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How to Cite
Dipak S. Kale. (2024). Prevalence of Beta-Lactamase Producing Genes in Escherichia Coli Isolated from Diabetic Foot Ulcers: A Hospital Based Study from South-Western Maharashtra. Revista Electronica De Veterinaria, 25(1), 355 - 367. Retrieved from