Melatonin Experiment: Exposing its Effects on Goat Physiology in Warming Conditions

  • Tanvi D, Vikram singh, Solomon Jebaraj
Keywords: Goats, melatonin, climate change, temperature, hormones, stress-associated genes, cell-protective effect


The welfare of cattle is threatened by climate change, especially in areas where temperatures are rising. The physiological traits of goats subjected to warmer temperatures are studied in this investigation to determine whether melatonin, a hormone renowned for regulating circadian cycles and stressful situations, can have any moderating impacts. Two goat groupings participated in a controlled study: Group 1 (G1) received melatonin nutrients, while Group 2 (G2) functioned as the untreated group. 50 female goats of the same weight and age were subjected to high heats (36°C and 42°C) for a total of five hours/day for five days in a row after being acclimated for four days at 26°C. During the course of the trial, 0.1 mg/kg of melatonin was injected at midday. Pre and post-melatonin treatment reactions are determined. The concentrations of hormones (T-4 and cortisol) and the proportion of each of genes associated with stress (ubiquitin and HSP-60) in mononuclear cells from the peripheral bloodstream have been evaluated in samples of blood drawn on different days. The findings demonstrated that as exposure temperatures rose in both G1 and G2, there was a substantial increase in rectal temperature and the rate of pulse. T-4 rates in the G1 decreased at 42°C, whereas cortisol levels in the G2 soared with exposure heat but kept decreasing in the G1. HSP-60 and other stress-associated genes were up-regulated in G1, especially at 42°C. In summary, reduced cortisol levels and increased expression of stress-associated genes suggested that melatonin had a cooling as well as cell-protective effect on goats in heated environments.


Muluneh, M.G., (2021). Impact of climate change on biodiversity and food security: a global perspective—a review article. Agriculture & Food Security, 10(1), pp.1-25. Doi:10.1186/s40066-021-00318-5

Zobel, G. and Nawroth, C., (2020). Current state of knowledge on the cognitive capacities of goats and its potential to inform species-specific enrichment. Small Ruminant Research, 192, p.106208. Doi: 10.1016/j.smallrumres.2020.106208

Silva, S.R., Sacarrão-Birrento, L., Almeida, M., Ribeiro, D.M., Guedes, C., Gonzalez Montana, J.R., Pereira, A.F., Zaralis, K., Geraldo, A., Tzamaloukas, O. and Cabrera, M.G., (2022). Extensive sheep and goat production: The role of novel technologies towards sustainability and animal welfare. Animals, 12(7), p.885. Doi: 10.3390/ani12070885

Gupta, M. and Mondal, T., (2021). Heat stress and thermoregulatory responses of goats: a review. Biological Rhythm Research, 52(3), pp.407-433. Doi: 10.1080/09291016.2019.1603692

Sejian, V., Silpa, M.V., Reshma Nair, M.R., Devaraj, C., Krishnan, G., Bagath, M., Chauhan, S.S., Suganthi, R.U., Fonseca, V.F., König, S. and Gaughan, J.B., (2021). Heat stress and goat welfare: Adaptation and production considerations. Animals, 11(4), p.1021.Doi: 10.3390/ani11041021

Ribeiro, M.N., Ribeiro, N.L., Bozzi, R. and Costa, R.G., (2018). Physiological and biochemical blood variables of goats subjected to heat stress–a review. Journal of Applied Animal Research, 46(1), pp.1036-1041. Doi: 10.1080/09712119.2018.1456439

Aleena, J., Sejian, V., Bagath, M., Krishnan, G., Beena, V. and Bhatta, R., (2018). The resilience of three indigenous goat breeds to heat stress based on phenotypic traits and PBMC HSP70 expression. International Journal of Biometeorology, 62, pp.1995-2005.Doi: 10.1007/s00484-018-1604-5

El-Tarabany, M.S., El-Tarabany, A.A. and Atta, M.A., (2017). Physiological and lactation responses of Egyptian dairy Baladi goats to natural thermal stress under subtropical environmental conditions. International Journal of Biometeorology, 61, pp.61-68. Doi: 10.1007/s00484-016-1191-2

Sarangi, S., (2018). Adaptability of goats to heat stress: A review. The Pharma Innovation Journal, 7(4), pp.1114-1126.

Habibu, B., Kawu, M.U., Aluwong, T. and Makun, H.J., (2017). Influence of seasonal changes on physiological variables, haematology and serum thyroid hormones profile in male Red Sokoto and Sahel goats. Journal of Applied animal research, 45(1), pp.508-516. Doi: 10.1080/09712119.2016.1220384

Shaji, S., Sejian, V., Bagath, M., Manjunathareddy, G.B., Kurien, E.K., Varma, G. and Bhatta, R., (2017). Summer season related heat and nutritional stresses on the adaptive capability of goats based on blood biochemical response and hepatic HSP70 gene expression. Biological Rhythm Research, 48(1), pp.65-83. Doi: 10.1080/09291016.2016.1232340

Rashamol, V.P., Sejian, V., Bagath, M., Krishnan, G., Archana, P.R. and Bhatta, R., (2020). Physiological adaptability of livestock to heat stress: an updated review. Journal of Animal Behaviour and Biometeorology, 6(3), pp.62-71. Doi: 10.31893/2318-1265jabb.v6n3p62-71

Singh, K.M., Singh, S., Ganguly, I., Nachiappan, R.K., Ganguly, A., Venkataramanan, R., Chopra, A. and Narula, H.K., (2017). Association of heat stress protein 90 and 70 gene polymorphism with adaptability traits in Indian sheep (OvisAries). Cell Stress and Chaperones, 22, pp.675-684. Doi: 10.1007%2Fs12192-017-0770-4

Gonzalez-Rivas, P.A., Chauhan, S.S., Ha, M., Fegan, N., Dunshea, F.R. and Warner, R.D., (2020). Effects of heat stress on animal physiology, metabolism, and meat quality: A review. Meat science, 162, p.108025. Doi: 10.1016/j.meatsci.2019.108025

Archana, P.R., Aleena, J., Pragna, P., Vidya, M.K., Niyas, A.P.A., Bagath, M., Krishnan, G., Manimaran, A., Beena, V., Kurien, E.K. and Sejian, V., (2017). Role of heat shock proteins in livestock adaptation to heat stress. J. Dairy Vet. Anim. Res, 5(1), p.00127. Doi: 10.1016/j.meatsci.2018.03.015

Pragna, P., Sejian, V., Soren, N.M., Bagath, M., Krishnan, G., Beena, V., Devi, P.I. and Bhatta, R., (2018). Summer season induced rhythmic alterations in metabolic activities to adapt to heat stress in three indigenous (Osmanabadi, Malabari and Salem Black) goat breeds. Biological Rhythm Research, 49(4), pp.551-565. Doi: 10.1080/09291016.2017.1386891

Abbas, Z., Sammad, A., Hu, L., Fang, H., Xu, Q. and Wang, Y., (2020). Glucose metabolism and dynamics of facilitative glucose transporters (GLUTs) under the influence of heat stress in dairy cattle. Metabolites, 10(8), p.312. Doi: 10.3390/metabo10080312

Slimen, I.B., Chniter, M., Najar, T. and Ghram, A., (2019). Meta-analysis of some physiologic, metabolic and oxidative responses of sheep exposed to environmental heat stress. Livestock Science, 229, pp.179-187. Doi: 10.1016/j.livsci.2019.09.026

Chauhan, S.S., Rashamol, V.P., Bagath, M., Sejian, V. and Dunshea, F.R., (2021). Impacts of heat stress on immune responses and oxidative stress in farm animals and nutritional strategies for amelioration. International journal of biometeorology, 65, pp.1231-1244. Doi: 10.1007/s00484-021-02083-3

Chen, D., Li, X., Zhao, X., Qin, Y., Wang, J. and Wang, C., (2019). Comparative proteomics of goat milk during heated processing. Food Chemistry, 275, pp.504-514. Doi: 10.1016/j.foodchem.2018.09.129

Basile, F., Capaccia, C., Zampini, D., Biagetti, T., Diverio, S. and Guelfi, G., (2020). Omics insights into animal resilience and stress factors. Animals, 11(1), p.47. Doi: 10.3390/ani11010047

Lim, C.L., (2020). Fundamental concepts of human thermoregulation and adaptation to heat: a review in the context of global warming. International Journal of Environmental Research and Public Health, 17(21), p.7795. Doi: 10.3390/ijerph17217795

Kant, L., Kittur, P.M., Kumar, A., Majumder, S., Rajawat, N., Samad, H.A., Chouhan, V.S., Singh, G. and Maurya, V.P., (2023). The impact of chronic heat stress on thyroid hormone dynamics in Sahiwal and Tharparkar cattle.

Vaughan, M.K. and Vaughan, G.M., (2020). Metabolic and thyroidal consequences of melatonin administration in mammals. In Melatonin (pp. 311-347). CRC Press. Doi: 10.1201/9781003068570-12

Bowman, C.E., Arany, Z. and Wolfgang, M.J., (2021). Regulation of maternal–fetal metabolic communication. Cellular and molecular life sciences, 78, pp.1455-1486. Doi:10.1007/s00018-020-03674-w

He, Y., Heumüller, T., Lai, W., Feng, G., Classen, A., Du, X., Liu, C., Li, W., Li, N. and Brabec, C.J., (2019). Evidencing excellent thermal‐and photostability for single‐component organic solar cells with inherently built‐in microstructure. Advanced Energy Materials, 9(21), p.1900409. Doi: 10.1002/aenm.201900409

Joy, A., Dunshea, F.R., Leury, B.J., Clarke, I.J., DiGiacomo, K. and Chauhan, S.S., (2020). Resilience of small ruminants to climate change and increased environmental temperature: A review. Animals, 10(5), p.867. Doi: 10.3390/ani10050867

Pincus, D., (2020). Regulation of Hsf1 and the heat shock response. HSF1 and molecular chaperones in biology and cancer, pp.41-50. Doi: 10.1007/978-3-030-40204-4_3

How to Cite
Tanvi D, Vikram singh, Solomon Jebaraj. (2024). Melatonin Experiment: Exposing its Effects on Goat Physiology in Warming Conditions. Revista Electronica De Veterinaria, 25(1), 86-94. Retrieved from