The Usage of Biancaea Sappan and Crocus Sativus as Natural Dyes for the Liver Fluke, Fasciola Gigantica

  • Andini Nurlaelasari Department of Animal Science, Sebelas Maret University, Surakarta 57126, Indonesia
  • A’isyah Retno Wulandari Department of Animal Science, Sebelas Maret University, Surakarta 57126, Indonesia
  • Dimas Ariyanto Department of Animal Science, Sebelas Maret University, Surakarta 57126, Indonesia
  • Penny Humaidah Hamid Department of Animal Science, Sebelas Maret University, Surakarta 57126, Indonesia
Keywords: Biancaea sappan; Crocus sativus; Fasciola gigantica


The herbs in Indonesia are rich in substances with medicinal effect as well as natural compounds for many purposes. The herbs are easily cultivated in the supporting climate and thus, guaranteeing it’s availability and sustainability. The aim of this study was to utilize Biancaea sappan and Crocus sativus as natural dye for Fasciola gigantica compared to commonly used dye, the acetocarmine. F. gigantica itself was frequently found as the liver fluke of cattle in Indonesia with significant economic losses. B. sappan and C. sativus were mashed and soaked in 100% glacial acetic acid overnight. The extracts were utilized to stain adult F. gigantica isolated from infected cattle in Ampel abattoir, Boyolali, Central Java, Indonesia. Acetocarmine 5.5% was a control during staining process. The results showed that structures and internal organs were pigmented in comparable degree with the control. Both of the B. sappan and C. sativus were successfully stained F. gigantica structures such as oral sucker, ventral sucker, uterine, eggs inside the uterine and testes as compared to acetocarmine. The pigmentation result showed that the C. sativus stained spina better than acetocarmine. The methods are eco-friendly and safe since the herbs do not have carcinogenic effect.


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How to Cite
Nurlaelasari, A., Wulandari, A. R., Ariyanto, D., & Hamid, P. H. (2023). The Usage of Biancaea Sappan and Crocus Sativus as Natural Dyes for the Liver Fluke, Fasciola Gigantica. Revista Electronica De Veterinaria, 24(1), 77 - 83. Retrieved from