Methicillin Resistant Staphylococcus aureus (MRSA) Isolated from Skin Infections in Poultry (Bumble Foot)

  • Basim Mohammed Hanon Department of Microbiology, College of Veterinary Medicine, University of Wasit, Wasit, Iraq
  • Alaa AM AL-Khafajy Department of Biology, College of Science, University of Wasit, Wasit, Iraq
Keywords: mecA gene, Polymerase chain reaction, Antimicrobial sensitivity testing, VITEK2 system, Biochemical testing, Iraq

Abstract

This study was aimed to isolate and identify of MRSA from infected skin of poultry, and testing the susceptibility of the isolates to different antibiotics especially methicillin, and confirmation of mecA gene encoded to methicillin resistance. Therefore, a total of 50 skin swabs were collected from the suspected poultry foot cases at different farm in Wasit province / Iraq, during November (2021) to January (2022). The swab samples were grown and purified, and then, the isolates were tested biochemically and phenotypically and subjected finally to antimicrobial sensitivity testing and molecular confirmation using conventional PCR assay. Examination of suspected S. aureus isolates revealed that the number of grown and fermented isolates on Mannitol salt agar (MSA) was 48%, and 12% for non-fermented MSA isolates; while, 40% of isolates were not grown on MSA. The colonies of suspected S. aureus were appeared on MSA as large, convex, shiny to opaque, circular, and golden-yellowish color; while on blood agar, colonies had signif, rounded, smooth, and grayish appearance. All grown and fermented isolates were showed positive reactivity to Catalase (100%) and Bound Coagulase (87.5%) test

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Agarose-gel electrophoresis (2%) in targeting the mecA gene
Published
2022-12-31
How to Cite
Hanon, B. M., & AL-Khafajy, A. A. (2022). Methicillin Resistant Staphylococcus aureus (MRSA) Isolated from Skin Infections in Poultry (Bumble Foot). Revista Electronica De Veterinaria, 23(4), 121 - 130. Retrieved from https://www.veterinaria.org/index.php/REDVET/article/view/287