Can Static Magnetic Field and Gingerol Improve Osteogenic Property?
Abstract
INTRODUCTION
Static magnetic fields (SMF), which have a constant magnetic field strength and direction, have a long history. Numerous studies show that exposure to mild SMF (1 mT) can boost bone density and mass. In recent years, researchers have been investigating alternative approaches to enhance bone formation and promote osteogenic properties. Osteoporosis and bone-related disorders pose significant health challenges worldwide, leading to fractures, impaired mobility, and reduced quality of life. Promoting osteogenesis, the process of bone formation is of great importance in developing strategies for bone tissue engineering and regenerative medicine. Gingerol is a bioactive compound found in ginger and is responsible for its characteristic flavor and aroma. Like ginger, gingerol has been studied for its potential effects on various aspects of health, including bone formation. Gingerol has been shown to increase the expression of genes related to osteoblast differentiation and mineralization, suggesting a potential role in promoting bone formation.
AIM AND OBJECTIVE
To study whether static magnetic field and gingerol in combination have a positive effect on the osteogenic property of human osteoblastic cells under in vitro
MATERIALS AND METHODS
Human MG63 cells were procured from NCCS Pune, India. The cells were maintained under normal culture conditions with 5% carbon dioxide and 10% FBS containing DMEL medium. The cells were trypsinized and passaged for further use. The viability and cell morphology were assessed to check the cell viability.
Treatment protocol:
The cells were exposed to 25 millitesla of static magnetic field by using neodymium magnets and simultaneously exposed to gingerol of 2.5 millimolar concentration for a period Again, for specific time points.
RESULT
This study shows that static magnetic fields and gingerol in combination improves the osteogenic property
CONCLUSION
Assessing the clinical applicability of static magnetic fields and gingerol in patients with compromised skeletal health, such as osteoporosis or bone fractures, is an important future direction. Conducting well-designed clinical trials with appropriate patient populations and outcome measures will help determine the therapeutic potential of these interventions.
References
2. Ravindran PN, Nirmal Babu K. Ginger: The Genus Zingiber. CRC Press; 2016. 576 p.
3. Sekaran S, Vimalraj S, Thangavelu L. The Physiological and Pathological Role of Tissue Nonspecific Alkaline Phosphatase beyond Mineralization. Biomolecules. 2021 Oct 21;11(11):1564.
4. Chauhan DN, Singh PR, Chauhan NS, Shah K. Pharmacological Studies in Natural Oral Care. John Wiley & Sons; 2023. 884 p.
5. Katiyar V, Gupta R, Ghosh T. Advances in Sustainable Polymers: Processing and Applications. Springer Nature; 2019. 483 p.
6. Re-appraising the role of flavonols, flavones and flavonones on osteoblasts and osteoclasts- A review on its molecular mode of action. Chem Biol Interact. 2022 Mar 1;355:109831.
7. Gnanasekaran D. Green Biopolymers and their Nanocomposites. Springer; 2019. 437 p.
8. Chakraborti S. Handbook of Oxidative Stress in Cancer: Therapeutic Aspects. Springer Nature; 2022. 4078 p.
9. Sukhani DK. Home/Ayurvedic Remedies for Coronavirus (COVID-19). MeetCoogle; 2020. 45 p.
10. Govindarajan D, Saravanan S, Sudhakar S, Vimalraj S. Graphene: A Multifaceted Carbon-Based Material for Bone Tissue Engineering Applications. ACS Omega [Internet]. 2023 Dec 21 [cited 2024 Jan 24]; Available from: https://pubs.acs.org/doi/full/10.1021/acsomega.3c07062
11. Idris AI. Bone Research Protocols. 2019. 670 p.
12. Banerjee D. 3D Printed Calcium Phosphate Scaffolds and Coatings on Titanium Alloys for Orthopedic Applications: Effects of Dopants and Natural Medicinal Compounds on Biological Properties. 2019. 296 p.
13. Saad B, Zaid H, Shanak S, Kadan S. Anti-diabetes and Anti-obesity Medicinal Plants and Phytochemicals: Safety, Efficacy, and Action Mechanisms. Springer; 2017. 257 p.
14. Layman J. Chu #1. Image Comics; 2020. 32 p.
15. Kintzios SE, Barberaki MG. Plants that Fight Cancer, Second Edition. CRC Press; 2019. 504 p.
16. Vimalraj S, Sekaran S. RUNX Family as a Promising Biomarker and a Therapeutic Target in Bone Cancers: A Review on Its Molecular Mechanism(s) behind Tumorigenesis. Cancers . 2023 Jun 19;15(12):3247.
17. Wallace T. Dietary Bioactives and Bone Health. 2018.
18. Li Y. Effects of Programmed Local Delivery from a Micro Nano-Hierarchical Surface on Titanium Implant on Infection Clearance and Osteogenic Induction in an Infected Bone Defect. 2017.
19. Tang LS, Qiu CZ, Zhang HY, Ren DL. Effects of 0.4 T, 3.0 T and 9.4 T static magnetic fields on development, behaviour and immune response in zebrafish (Danio rerio). Neuroimage. 2023 Nov 15;282:120398.
20. Lim TK. Edible Medicinal and Non-Medicinal Plants: Volume 12 Modified Stems, Roots, Bulbs. Springer; 2016. 702 p.
21. Liu H, Wang Z, Li X, Zhao B, Li H, Han L, et al. CeO Nanoparticle Bioactive Materials Promote MG-63 Osteogenic Differentiation and Antioxidant Activity Through NRF2 Signaling. Appl Biochem Biotechnol [Internet]. 2023 Nov 10; Available from: http://dx.doi.org/10.1007/s12010-023-04766-6
22. Zhang B, Feng J, Chen S, Liao R, Zhang C, Luo X, et al. Cell response and bone ingrowth to 3D printed Ti6Al4V scaffolds with Mg-incorporating sol-gel TaO coating. RSC Adv. 2023 Nov 7;13(47):33053–60.
23. Hwang JS, Won SJ, Gong HS. How Does the Subchondral Bone Density Distribution of the Distal Humerus Change Between Early and Advanced Stages of Osteoarthritis? Clin Orthop Relat Res [Internet]. 2023 Nov 15; Available from: http://dx.doi.org/10.1097/CORR.0000000000002921
24. Selvaraj V, Sekaran S, Rajamani Sekar SK. Surgical intervention as a driver of new angiogenesis in tumors-time to consider minimally invasive surgeries? Int J Surg. 2023 Oct 1;109(10):3222–3.
25. Dosu G, Obanla TO, Zhang S, Sang S, Adetunji AO, Fahrenholz AC, et al. Supplementation of ginger root extract into broiler chicken diet: effects on growth performance and immunocompetence. Poult Sci. 2023 Oct;102(10):102897.
