Isolation, Morphological Identification and Invitro Antibacterial Activity of Crude Ink and Mucus of Dolabella Auricularia, Wedge Sea Hare (Light Foot 1786)
Keywords:
D.auricularia, Invitro, Ethanolic extract, Antibacterial activity
Abstract
The formative research focuses on the isolation, morphological identification and invitro antibacterial properties of ethanolic crude extracts of ink and mucus derived from sea hare D.auricularia a marine gastropod against selected human pathogenic bacteria. Using the disk diffusion method, antibacterial activity evaluated and the inhibition zones ranged from 5–10 mm for ink and 6–8 mm for mucus, with Staphylococcus aureus showing the highest susceptibility (10 mm). Compared to ciprofloxacin (28–33 mm), D.auricularia extracts were less effective. Bacillus cereus exhibited resistance to mucus extract. These findings suggest sea hare D.auricularia as a potential source of therapeutic agents, requiring further investigation to isolate bioactive compounds for pharmaceutical applications.
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9. Exner, M.; Bhattacharya, S.; Christiansen, B.; Gebel, J.; Goroncy-Bermes, P.; Hartemann, P.; Heeg, P.; et al. (2017). Antibiotic resistance: What is so special about multidrug-resistant Gram-negative bacteria? GMS hygiene and infection cont., 12: Doc05.
10. Hassan A. H. Ibrahim, Mohamed S. Amer, Hamdy O. Ahmed and Nahed A.
11. Hassan 2020. Antimicrobial activity of the sea hare (Aplysia fasciata) collected from the Egyptian Mediterranean Sea, Alexandria.
12. Ibrahim et al., 2020 246 Ibrahim, H.A.H.; Elshaer, M.M.; Elatriby, D.E. and Ahmed, H.O. (2020). Antimicrobial activity of the sea star (Astropecten spinulosus) collected from the Egyptian Mediterranean Sea, Alexandria. Egy. J. Aqua. Biol. Fish., 24(2): 507-523.
13. Maripandi, A., L. Prakash, Ar. Satamah, 2010. HPTLC separation of antibacterial compound from Perna virdis and Portunus sanguinolentus and its activity tested against common bacterial pathogens.
14. Melo, V.M., Fonseca, A.M., Vasconcelos, I.M., & Carvalho, A.F. (1998). Toxic, antimicrobial and hemagglutinating activities of the purple fluid of the sea hare Aplysia dactylomela Rang, 1828. Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas, 31 6, 785-91.
15. Nocchi, N.; Soares, A.R.; Souto, M.L. and Pereira, R.C. (2017). Detection of a chemical cue from the host seaweed Laurencia dendroidea by the associated mollusc Aplysia brasiliana. PLoS One, 12(11): e0187126.
16. Pereira RB, Pereira DM, Jiménez C, Rodríguez J, Nieto RM, Videira RA, Silva O, Andrade PB, Valentão P. Anti-Inflammatory Effects of 5α,8α-Epidioxycholest-6-en-3β-ol, a Steroidal Endoperoxide Isolated from Aplysia depilans, Based on Bioguided Fractionation and NMR Analysis. Mar Drugs. 2019 Jun 3;17(6):330.
17. S N Sethi and Mahadev Rama Kokane and Guneswar Sethi, 2019. Occurrence of Mottled Sea Hare, Aplysia fasciata Poiret, 1789 from Pulicat Lake, Tamil Nadu, India.
18. Sharma D, Patel RP, Zaidi STR, Sarker MMR, Lean QY, Ming LC. 2017.
19. Interplay of the quality of ciprofloxacin and antibiotic resistance in developing countries. Front Pharmacol 8: 1-7.
20. Singh AK, Sharma RK, Sharma V, Singh T, Kumar R, Kumari D. Isolation, morphological identification and in vitro antibacterial activity of endophytic bacteria isolated from Azadirachta indica (neem) leaves. Vet World. 2017 May;10(5):510-516.
Published
2024-06-27
How to Cite
S. Tamil Selvi, K. Bhanumathi, & M. Sasirekhamani. (2024). Isolation, Morphological Identification and Invitro Antibacterial Activity of Crude Ink and Mucus of Dolabella Auricularia, Wedge Sea Hare (Light Foot 1786). Revista Electronica De Veterinaria, 25(1), 3951-3955. https://doi.org/10.69980/redvet.v25i1.1949
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