Revealing of Aerolysin and Cytotoxic enterotoxin genes in Aeromonas hydrophila isolated from (Cyprinus carpio) fish in Mosul, Iraq
The research aimed on recovering Aeromonas hydrophila bacteria from the common carp fish (Cyprinus carpio) in Mosul city and study of its virulence by Molecular detection of Aerolysin (aer) and Cytotoxic Enterotoxin (alt) genes. A total of 100 fish samples with obvious signs of illness examined. Of the 134 isolates obtained from gills, air sac, skin and intestine 45 (34%) were identified bacteriologically as Aeromonas hydrophila. The ferocity of the isolates was investigated by using particular primers for each of the virulence (aer) and (alt) genes, the results revealed at least 2 of these genes in 36 (80 %) isolate. It is clarified that the major of the strains under exam possessed one or more genes coding virulence. These consequences confirm that A. hydrophila obtained from carp fish possess virulence factors and may be a source of infections threating such important industry.