Skin Fibrosarcoma and Chloracne Induced by (Acute and Long Term) Effects of 2,3,7,8- Tetrachlorodibenzo-p-Dioxin (TCDD) in Male Guinea Pig (Pathological and Biochemical Assay)
In the present study, we investigated the effects of acute and & long term exposure orally administrated to TCDD (2,3,7,8- tetrachlorodibenzo-p-dioxin) male Hartley guinea pigs ( n= 42 ) were randomly divided into 3 groups: 1st group ( control ) kept as control and given corn oil it consists 14 animals, 2nd group ( n= 14 ) was orally administrated at dose 1 μg /kg BW by gavage TCDD mixed with 1 ml/kg BW acetone – corn oil for 7 days, 3rd group ( n= 14 ) orally weekly administered by gavage 0.008 μg /kg BW TCDD mixed with 1 ml/kg BW acetone – corn oil for 6 months. In addition, skin samples about from 1 cm3 from neck, head & abdominal taken half of all on day 7 & after 6 months for biochemical analysis to thiobarbituric determination acid reactive substances ( TBARS ), catalase ( CAT ) and cuzen-SOD level by spectrophotometric methods, the other half skin sample were taken for histopathological analysis.
Results showed that 2nd group & 3rd group TCDD significantly (P<0.05) increased in TBARS & decreased in CAT & Cuzen-SOD level, pathological changes indicated at days 7 & after 6 months elevated nodules irregular on the skin of face, neck & abdomen with rough irregular dry hair & histopathological changes indicated at 7 days ( acute group ) chloracne acne-like lesion characterized by hyperkeratosis, acanthosis & at 6 months multinucleated giant cell with pleomorphic nuclei & fibrosarcoma tumor.
In conclusion, TCDD caused oxidative stress in a time-dependent manner & chloracne lesion mostly in acute TCDD administration & fibrosarcoma in the long term TCDD administration.