Estimation Of Praziquantel In Tablets By UV Visible Spectrophotometry And Its Forced Degradation Studies
Abstract
The aim of the project is to create a simple and inexpensive UV visible spectrophotometric method that can be validated in accordance with ICH guidelines for the estimation of praziquantel in bulk and tablet dosage form. The technique used the absorbance maxima method, which is based on the measurement of praziquantel's absorbance at 261.8 nm in ethanol. According to ICH guidelines, the method was validated for linearity, precision, accuracy, LOD, and LOQ. For praziquantel, the proposed technique was found to be linear over the concentration range of 10-950 µg/ml. In accordance with ICH guidelines, it was put under various stress conditions. A stability-indicating UV visible spectroscopic method was developed for the analysis of the drug in the presence of the degradation products. It involved a 5-hour study, in which distilled ethanol was used as a solvent. The amount of degraded drug was calculated by taking absorbance at 261.8 nm.
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