Mejoramiento en la detección y cuantificación del adenovirus aviar de tipo 4 en tejido de hígado de pollo mediante la optimización de la PCR
Abstract
Fowl adenovirus type 4 (FAdV-4) is a highly prevalent pathogen affecting poultry worldwide, causing significant economic losses in the industry. In this study, we aimed to detect and quantify FAdV-4 in chicken liver tissue samples using an optimized quantitative polymerase chain reaction (qPCR) technique. Through the analysis of 962 samples collected between 2016 and 2018, we determined a prevalence rate exceeding 58% of FAdV-4 in the studied population. Additionally, sequencing analysis revealed a high similarity (99.43%) of the positive samples to the HLJDAd15 strain. Our findings emphasize the importance of implementing stringent measures to control and prevent the spread of FAdV-4, given its significant impact on poultry health and production. The optimized qPCR technique provides a reliable and efficient means for the detection and quantification of FAdV-4, enabling early diagnosis and appropriate management strategies. These results contribute to the understanding of FAdV-4 epidemiology and highlight the need for continued surveillance and control measures in poultry farming systems.
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