Colchicine-Induced Chromosomal Aberrations and Mitotic Inhibition in Allium sativum var sativum Root Meristems

  • Nalini T J
  • Prathibha K Y.
  • Geethanjali R.
  • Aishwarya J.
  • Keziah Tryphosa M.
  • Marhoob Banu UG student of Botany and Biotechnology, Maharani Cluster University, Palace Road, Bengaluru, Karnataka, India 560001
DOI: https://doi.org/10.69980/redvet.v25i1.1389
Keywords: Allium sativum var sativum, colchicine, chromosomal aberrations, mitosis inhibition, metaphase arrest, c-metaphase, anaphase bridges, polyploidy, cytogenetics

Abstract

Allium sativum, commonly known as garlic, is a perennial flowering plant that develops from a bulb. Colchicine acts as a microtubule-destabilizing agent and is a plant metabolite. This study explores the effects of colchicine on mitosis and chromosomal behavior in Allium sativum var sativum (garlic) root apical meristem cells. Garlic cloves were treated with varying concentrations of colchicine (0.5%, 1.0%, and 1.5%) to observe the impact on mitotic inhibition and metaphase arrest. Root tips were processed and examined microscopically, revealing a range of chromosomal abnormalities. These included c-metaphase, anaphase bridges, vagrant and laggard chromosomes, chromosomal stickiness, and multinucleated cells. The frequency and severity of these abnormalities increased with higher colchicine concentrations. Observations also indicated the presence of polyploid cells, nuclear lesions, and distorted cell division stages. The study highlights the dose-dependent effects of colchicine in disrupting normal cell division processes in Allium sativum var sativum contributing to a deeper understanding of its role in cytogenetic research.

Author Biographies

Nalini T J

Associate Professors Department of Botany, Maharani Cluster University, Palace Road, Bengaluru, Karnataka, India 560001

 

Prathibha K Y.

Professor Department of Botany, Maharani Cluster University, Palace Road, Bengaluru, Karnataka, India 560001

Geethanjali R.

Associate Professors Department of Botany, Maharani Cluster University, Palace Road, Bengaluru, Karnataka, India 560001

Aishwarya J.

UG student of Botany and Chemistry, Maharani Cluster University, Palace Road, Bengaluru, Karnataka, India 560001

 

Keziah Tryphosa M.

UG student of Botany and Biotechnology, Maharani Cluster University, Palace Road, Bengaluru, Karnataka, India 560001

Marhoob Banu, UG student of Botany and Biotechnology, Maharani Cluster University, Palace Road, Bengaluru, Karnataka, India 560001

student of Botany and Zoology, Maharani Cluster University, Palace Road Bengaluru, Karnataka, India 560001

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Published
2024-11-25
How to Cite
Nalini T J, Prathibha K Y., Geethanjali R., Aishwarya J., Keziah Tryphosa M., & Marhoob Banu. (2024). Colchicine-Induced Chromosomal Aberrations and Mitotic Inhibition in Allium sativum var sativum Root Meristems. Revista Electronica De Veterinaria, 25(1), 2828 -2840. https://doi.org/10.69980/redvet.v25i1.1389
Issue
Vol. 25 No. 1 (2024)
Section
Articles