Development And Validation Of Hplc Method For The Simultaneous Estimation Of Curcumin And Metformin In Pharmaceutical Dosage Form
Abstract
Introduction: Curcumin and Metformin are two well-known drugs which have anti-inflammatory and anti- diabetic property respectively but when used in combination they are known to be potent anticancer agents. It was studied that a combination of metformin and curcumin induces the apoptosis without affecting the cell cycle in LNCaP prostate cancer cell line.
Materials and methods: High profile liquid chromatography determination was performed with Agilent Technologies Model 1220 Infinity II LC Software Open Lab, VWD Type Lamp, flow rate was set to 1 ml/min and acetonitrile: water in the ratio 70:30 was used as mobile phase and the effluents were analyzed at 235 nm and 425 nm for metformin and curcumin respectively.
Results and discussion: In the concentration range of 2-10 µg/ml, the HPLC method demonstrated linearity for both curcumin and metformin, with correlation coefficients (r²) of 0.9953 and 0.9949 at 425 nm and 235 nm, respectively. Data on precision, robustness, and ruggedness revealed %RSD <2%.
Conclusion: the developed HPLC method were found to be simple, precise, specific and sensitive for the simultaneous estimation of curcumin and metformin
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