Development And Validation Of Hplc Method For The Simultaneous Estimation Of Curcumin And Metformin In Pharmaceutical Dosage Form

  • Sahil Nadaf
  • Akash Bakale
  • Sushmita I. Hiremath
  • Akshata P. Hebballi
  • Basavaraja Pujar
  • Ashika M.V
  • Sanjana Vadavalli
  • Akshata Mensinakai
  • Suresh.S Honnalli
Keywords: Curcumin, Metformin, Method development, Validation

Abstract

Introduction: Curcumin and Metformin are two well-known drugs which have anti-inflammatory and anti- diabetic property respectively but when used in combination they are known to be potent anticancer agents. It was studied that a combination of metformin and curcumin induces the apoptosis without affecting the cell cycle in LNCaP prostate cancer cell line.

Materials and methods: High profile liquid chromatography determination was performed with Agilent Technologies Model 1220 Infinity II LC Software Open Lab,  VWD Type Lamp, flow rate was set to 1 ml/min and acetonitrile: water in the ratio 70:30 was used as mobile phase and the effluents were analyzed at 235 nm and 425 nm for metformin and curcumin respectively.

 Results and discussion: In the concentration range of 2-10 µg/ml, the HPLC method demonstrated linearity for both curcumin and metformin, with correlation coefficients (r²) of 0.9953 and 0.9949 at 425 nm and 235 nm, respectively. Data on precision, robustness, and ruggedness revealed %RSD <2%.

Conclusion:  the developed HPLC method were found to be simple, precise, specific and sensitive for the simultaneous estimation of curcumin and metformin

Author Biographies

Sahil Nadaf

Department of Pharmaceutical Chemistry, KLE College of Pharmacy, Vidyanagar, Hubli-580031 (A Constituent Unit of KAHER, Belagavi)

Akash Bakale

Department of Pharmaceutical Chemistry, KLE College of Pharmacy, Vidyanagar, Hubli-580031 (A Constituent Unit of KAHER, Belagavi)

Sushmita I. Hiremath

Department of Pharmaceutical Chemistry, KLE College of Pharmacy, Vidyanagar, Hubli-580031 (A Constituent Unit of KAHER, Belagavi)

Akshata P. Hebballi

Department of Pharmaceutical Chemistry, KLE College of Pharmacy, Vidyanagar, Hubli-580031 (A Constituent Unit of KAHER, Belagavi)

Basavaraja Pujar

Department of Pharmaceutical Chemistry, KLE College of Pharmacy, Vidyanagar, Hubli-580031 (A Constituent Unit of KAHER, Belagavi)

Ashika M.V

Department of Pharmaceutical Chemistry, KLE College of Pharmacy, Vidyanagar, Hubli-580031 (A Constituent Unit of KAHER, Belagavi)

Sanjana Vadavalli

Department of Pharmaceutical Chemistry, KLE College of Pharmacy, Vidyanagar, Hubli-580031 (A Constituent Unit of KAHER, Belagavi)

Akshata Mensinakai

Department of Pharmaceutical Chemistry, KLE College of Pharmacy, Vidyanagar, Hubli-580031 (A Constituent Unit of KAHER, Belagavi)

Suresh.S Honnalli

Department of Pharmaceutical Chemistry, KLE College of Pharmacy, Vidyanagar, Hubli-580031 (A Constituent Unit of KAHER, Belagavi)

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Published
2024-09-09
How to Cite
Sahil Nadaf, Akash Bakale, Sushmita I. Hiremath, Akshata P. Hebballi, Basavaraja Pujar, Ashika M.V, Sanjana Vadavalli, Akshata Mensinakai, & Suresh.S Honnalli. (2024). Development And Validation Of Hplc Method For The Simultaneous Estimation Of Curcumin And Metformin In Pharmaceutical Dosage Form. Revista Electronica De Veterinaria, 25(1), 1933 -1942. https://doi.org/10.69980/redvet.v25i1.1038
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Articles