Effect of semen dilution and equilibration time on semen quality of Pesisir bull
Abstract
The process of freezing semen, a common problem, is generally caused by cold shock to frozen cells and the formation of ice crystals. One way to overcome this is to look for diluents and optimal equilibration times to reduce damaged spermatozoa during freezing. This study aims to determine the semen quality of Pesisir Bull and the interaction between the two types of diluent and the four equilibration times. The diluent used was egg yolk tris and andromed. The equilibration times were 6, 8, 10, and 12 hours. The variables observed included macroscopic and microscopic semen evaluation. This study used a factorial randomized block design with two factors and four treatment replications. Data are reported as the mean and standard deviation analyzed using analysis of variance. The average percentage of semen motility before equilibration was 80±0.00; after equilibration, 60.00±0.00 up to 72.50±0.00. The mean percentage of viability before equilibration was 89.26±2.70 after equilibration, which was in the range of 65.44±2.72 to 78.78±2.99. The average rate of abnormalities before equilibration was 3.00±0.50 after equilibration, which was in the field of 5.81±1.29 up to 11.41±1.63. The mean percentage of IPM before equilibration was 84.18±1.18 after equilibration, which was 71.37±3.72 up to 77.85±3.53. The average rate of IAC before equilibration was 87.84±3.14 after equilibration, which was 77.69±3.65 up to 83.07±2.11. The egg yolk tris diluent treatment showed highly significant (P<0.01) differences with the andromed diluent treatment in motility, viability, IPM, and IAC. We concluded that the Semen of Pesisir Bull equilibrated from 6 to 12 hours using Andromed and egg yolk tris diluent is still categorized as suitable for further processing to manufacture frozen semen or for artificial insemination (AI).
References
Y. Yetmaneli, B. Purwanto, R. Priyanto, and W. Manalu, "Iklim Mikro dan Respon Fisiologis Sapi Pesisir di Dataran Rendah dan Dataran Tinggi Sumatera Barat," Jurnal Agripet, vol. 20, no. 2, 2020.
T. Afriani, F. Rahim, M. Mundana, A. Rahmat, and A. Farhana, "The Effect of GnRH on Reproductive Performance and Progesterone Hormone Levels in Buffalo in West Sumatera," Jurnal Triton, vol. 11, no. 2, pp. 1-8, 2020.
S. N. Sirajuddin, I. Sudirman, and L. D. Bahar, "Relationship between breeder characteristics and adoption of artificial insemination in Bali cattle," European Journal of Sustainable Development, vol. 7, no. 3, pp. 143-143, 2018.
R. Indriastuti, M. Ulum, R. Arifiantini, and B. Purwantara, "Individual variation in fresh and frozen semen of Bali bulls (Bos sondaicus)," Veterinary World, vol. 13, no. 5, p. 840, 2020.
M. Sharafi, S. M. Borghei-Rad, M. Hezavehei, A. Shahverdi, and J. D. Benson, "Cryopreservation of Semen in Domestic Animals: A Review of Current Challenges, Applications, and Prospective Strategies," Animals, vol. 12, no. 23, p. 3271, 2022.
N. S. Juyena and C. Stelletta, "Seminal plasma: an essential attribute to spermatozoa," Journal of andrology, vol. 33, no. 4, pp. 536-551, 2012.
N. Salmah et al., "The Motility, Viability, and Abnormality of the Frozen Bali Bull Semen with Andromed and Egg yolk-Tris Extender: Quality of Bali Bull Semen," Hasanuddin Journal of Animal Science (HAJAS), vol. 3, no. 1, pp. 8-14, 2021.
E. M. Murphy, B. Eivers, C. M. O'Meara, P. Lonergan, and S. Fair, "Effect of increasing equilibration time of diluted bull semen up to 72 h prior to freezing on sperm quality parameters and calving rate following artificial insemination," Theriogenology, vol. 108, pp. 217-222, 2018.
A. Fleisch et al., "Effects of an extension of the equilibration period up to 96 hours on the characteristics of cryopreserved bull semen," Theriogenology, vol. 89, pp. 255-262, 2017.
G. S. Bustani and F. H. Baiee, "Semen extenders: An evaluative overview of preservative mechanisms of semen and semen extenders," Veterinary World, vol. 14, no. 5, p. 1220, 2021.
T. Susilawati, Pedoman inseminasi buatan pada ternak. Universitas Brawijaya Press, 2013.
A. Baharum, R. I. Arifiantini, and T. L. Yusuf, "Freezing capability of Pasundan bull sperm using tris-egg yolk, tris-soy, and AndroMed® diluents," Jurnal Kedokteran Hewan-Indonesian Journal of Veterinary Sciences, vol. 11, no. 1, pp. 45-49, 2017.
M. Mukhlis, D. Dasrul, and S. Sugito, "Analisis motilitas spermatozoa sapi Aceh setelah pembekuan dalam berbagai konsentrasi andromed®," Jurnal Agripet, vol. 17, no. 2, pp. 112-120, 2017.
N. Setiono, S. Suharyati, and P. E. Santosa, "Brahman Bull’s Frozen Semen Quality with Differen Doses of Cryprotectant Glycerol in Yolk Tris Sitrat Diluent," Jurnal Ilmiah Peternakan Terpadu, vol. 3, no. 2, pp. 61-69, 2015.
T. Susilawati, Spermatology. Universitas Brawijaya Press, 2011, p. 171.
A. M. Mahmoud, B. Depoorter, N. Piens, and F. H. Comhaire, "The performance of 10 different methods for the estimation of sperm concentration," Fertility and sterility, vol. 68, no. 2, pp. 340-345, 1997.
W. Bebas, I. W. Gorda, K. K. Agustina, and I. M. Merdana, "Effects of Adding Glutathione to AndroMed Diluent on Intact Plasma and Acrosome Membranes and Progressive Motility of Cattle Spermatozoa During Freezing Processes," World's Veterinary Journal, no. 4, pp. 561-570, 2023.
E. Hafez, "Semen Evaluation in Reproduction in Farm Animal. 7th," Edition Lippincont Wiliams and Wilkins. Maryland, USA, 2000.
M. Toelihere, "Inseminasi Buatan pada Ternak. Angkasa. Bandung.," Theriogenology, vol. 38, pp. 209-222, 1993.
M. Iswadi and Z. F. Ann, "Morphometry of head spermatozoa of sexually matured Malayan Gaur Bulls (Bos gaurus hubbacki)," J. Trop. Agric and Fd. Sc, vol. 44, no. 2, pp. 315-324, 2016.
I. Budhiyadnya, Z. Udin, E. Purwati, and Y. Yellita, "The effect of age, body height, weight, testosterone hormone concentration and semen quality on the libido level of pesisir cattle," J. Anim. Health Prod, vol. 9, no. 1, pp. 78-87, 2021.
A. Shahverdi, A. Rastegarnia, and T. R. Topraggaleh, "Effect of extender and equilibration time on post thaw motility and chromatin structure of buffalo bull (Bubalus bubalis) spermatozoa," Cell Journal (Yakhteh), vol. 16, no. 3, p. 279, 2014.
G. Sansone, M. Nastri, and A. Fabbrocini, "Storage of buffalo (Bubalus bubalis) semen," Animal reproduction science, vol. 62, no. 1-3, pp. 55-76, 2000.
Y. Mariani and A. Alimuddin, "Penambahan Level Ekstrak Wortel (Daucus carota) pada Pengencer Andromed Dalam Mempertahankan Kualitas Spermatozoa Sapi Bali pada Suhu 5 OC," JURNAL SAINS TEKNOLOGI & LINGKUNGAN, vol. 6, no. 2, pp. 241-248, 2020.
F. Hoesni, "Efek penggunaan susu skim dengan pengencer tris kuning telur terhadap daya tahan hidup spermatozoa sapi," Jurnal Ilmiah Universitas Batanghari Jambi, vol. 16, no. 3, pp. 46-56, 2016.
F. X. Manehat, A. A. Dethan, and P. K. Tahuk, "Motility, viability, spermatozoa abnormality, and ph of bali cattle semen in another-yellow water driller stored in a different time," Journal of Tropical Animal Science and Technology, vol. 3, no. 2, pp. 76-90, 2021.
R. Arifiantini and H. Maheswari, "Kualitas Semen Cair Sapi dalam Pengencer Tris Kuning Telur yang Diberi Ekstrak Daun Kelor (Moringa oleifera)."
S. Sitepu and J. Marisa, "Percentage value of membrane integrity and acrosome integrity spermatozoa in simmental liquid semen with addition penicillin and sweet orange essential oil," in IOP Conference Series: Earth and Environmental Science, 2019, vol. 327, no. 1: IOP Publishing, p. 012027.
S. Sitepu and U. Zaituni, "Improved quality of frozen boer goat semen with the addition of sweet orange essential oil on tris yolk and gentamicin extender," in IOP Conference Series: Earth and Environmental Science, 2018, vol. 122, no. 1: IOP Publishing, p. 012125.
