In Vitro Effectiveness Evaluation of Eco-Enzyme to Inhibit the Growth of Aspergillus sp. Fungi that Isolated from Dog’s Skin
Abstract
Dermatitis is an inflammation of the skin that often occurs in dogs. The fungus of Aspergillus sp. is reported to be one of the causes of dermatitis. Eco-enzyme is a herbal substance believed to be used as an antifungal. The eco-enzyme in this study were made from neem leaves (Azadirachta indica A. Juss), papaya peels (Carica papaya L.), soursop peels (Annona muricata L.), and citronella (Cymbopogon nardus L.). This study aims to determine the inhibitory power of eco-enzyme in inhibiting the growth of Aspergillus sp. isolated from a dog’s epidermis. This study used a quantitative method with modification of agar plate diffusion (Kirby Bauer) with well diffusion techniques. This experimental study used a completely randomized design with six treatments; eco-enzyme concentrations of 25%, 50%, 75%, 100%, positive control, and negative control. The effectiveness of eco-enzyme was determined by observing and calculating the inhibition zones formed on SDA media. Data analysis used ANOVA and continued with the Games- Howell test. The results showed that eco-enzyme made from neem leaf extract, papaya peels, soursop peels, and citronella effectively inhibited the growth of Aspergillus sp. at 25%, 50%, 75%, and 100%. It is necessary to test eco-enzyme effectiveness in dogs infected with Aspergillus sp. using in vivo testing to see the ability to inhibit its growth.
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