Exploring the Protective Effect of Donkey Milk against Indomethacin-Induced Gastric Damage in a Rat Model
Abstract
The adverse effects of the anti-inflammatory medication indomethacin on the stomach mucosa are well-documented and well-understood. Due to its antioxidant characteristics, high antibody concentration, and minimal allergic traits, donkey milk has lately gained attention as a nutritional supplement. In this research, we looked at Exploring the Protective Effect of Donkey Milk against Indomethacin-Induced Gastric Damage in a Rat Model. The rats were divided into four categories. Standard rat chow was given to the first two categories of rats, whereas 25 mg/kg of donkey milk was given to the third and fourth categories daily through nasogastric gavage. Oral treatment of 30 mg/kg indomethacin on day 11 caused gastric mucosal injury in rats in categories 3 and 4. After 6 hours, every rat was euthanized, and their stomachs were collected for microscopic as well as biochemical analysis of malondialdehyde (MDA) and glutathione (GSH) levels. Immunohistochemistry was used to examine TNF- α expression in the stomach lining. The entire erosion area and the degree of linear ulceration were substantially reduced in the donkey milk-indomethacin category compared to the conventional food-indomethacin category (p <0.05). Additionally, this cohort showed considerable improvements in reduced MDA and increased GSH. Expression of tumour necrosis factor-alpha was greater and more constant in the gastritis group than in the donkey milk group. Donkey milk was shown to have significant protective effects against indomethacin-induced gastrointestinal damage.
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