Using Commercial Cryopreservation of Dromedary Camel Semen Using Tris-Based Extenders (T-BE), Free-From and Enriched With Egg Yolks
Abstract
Using commercial Tris-based extenders, the effectiveness of cryopreserving Semen from seven adult dromedary camels using both eggs yolk-supplemented and egg yolk-free methods were compared in this study. Control was provided via the SHOTOR camel-specific extension. AndroMed, OPTIXcell, Triladyl, Steridyl, SHOTOR, and Triladyl were employed to assess and dilute the collected semen samples. Before liquid nitrogen freezing, the dilute Two hours were spent progressively cooling and balancing the Semen. Using computer-assisted sperm analysis, the DNA fragmentation, anomalies, plasma membrane integrity, kinetics, vitality, and motility of the Semen were assessed both before and following freeze-thaw cycles. Progressively active sperm during the pre-freezing examination was greater in samples diluted with SHOTOR in data diluted with Steridyl, OPTIXcell, or AndroMed.Additionally, SHOTOR and Triladyl produced sperm with much higher viability and integrity of DNA than any other diluent, whereas Triladyl had sperm with significantly higher plasma membrane integrity. Triladyl significantly increased sperm motility in the post-thawing examination as opposed to SHOTOR, Steridyl, or AndroMed, with OPTIXcell as the lowest effective. The use of Triladyl produced the highest level of progressive sperm motility. AndroMed and Triladyl created the highest sperm velocities in curved, straight, and average routes after thawing, respectively. Triladyl generated sperm with the highest coefficients of linearity and straightness, while SHOTOR produced sperm with the greatest DNA and cell membrane integrity. Steridyl was less effective than Triladyl, while OPTIXcell and AndroMed led to subpar sperm quality after freezing viability. With OPTIXcell, the rate of aberrant sperm was highest.In contrast, SHOTOR or Triladyl had the lowest rate—however, the greatest sperm quality after Triladyl, SHOTOR, and semen cryopreservation provided thawing. Steridyl, AndroMed, and OPTIXcell are further viable treatments. Our research shows that Triladyl is currently the best commercially available extender for cryopreserving dromedary camel’s Semen.
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