Histopathological Changes in Testicular Tissues of Male Rabbits by Trimetion
Abstract
This study was conducted to investigate pathological lesions associated with Trimetion intoxication on testes and epididymis and fertility in adult male rabbits. Totally, 24 local male rabbits were purchased, prepared and randomly divided equally into four groups; (G1) negative control group, (G2) drenched Trimetion at 10 ml/Kg daily for two months; (G3) drenched Trimetion at 25 ml/Kg daily for two months; and (G4) drenched Trimetion at 50 ml/Kg daily for two months using of oral gavage. In contrast to control group, the testes of the treated groups had atrophy. The primary histopathological lesions of drenched rabbits revealed that Trimetion causes a dose-related damage to testes as evidenced by mild hydropic degeneration, sloughing of cell lining and by partial suppression of spermatogenesis in G1; while in G2, epididymis appears to be lack of sperm. In G3, there were more severe testicular damages with little hydropic degeneration, spermatogonial inhibition, and severe lack of spermatogenesis. The findings of G4 had aggressive, severe, and widespread lesions in all testicular tissue, severe necrosis and full spermatogonial suppression with no spermatogonia. In conclusion, the current study showed that the Trimetion is caused different pathological lesions in male testicular and epididymal tissues of adult rabbits which appeared microscopically sever necrosis and degenerations and complete suppression of spermatogenesis with vaculation of spermatogonia. However, moreover experiments are required to identify other toxicopathological changes in other body tissues.
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