Efficiency of Purified Biosurfactant (Pseudofactin) from P. Aeruginosa in Disruption of Biofilm Formation by Candida Albicans in Vaginal Candidiasis
Abstract
Candidiasis caused by Candida albicans is one of the most common forms of hospital-acquired opportunistic infection. So that the biosurfactant was detected in P. aeruginosa isolated from burns and wounds infections and produced with different levels of productivity. The use of (2:1) of chloroform: acetone was the best for extraction of biosurfactant with 11.3 g/l while chloroform: propanol gave a low yield of 2.41 g/l of biosurfactant. Candida albicans causing vaginal candidiasis considered a stronger producer of biofilms. The effect of biosurfactant on Candida biofilm formation was inhibited with increasing incubation period and the percentage of biofilm inhibition reached 45-78% after 24 hours and increased to 65-84% after 48 hours.
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