Molecular Characterization of Cysticercus tenicollis from Slaughtered Sheep and Goats in Basrah Province, Iraq
Abstract
Taenia hydatigenia refers as cestoda in carnivorous species, whereas Cysticercus tenicollis is a common larval stage found in herbivorous animals, ruminants and swine. An investigation into molecular identification of C. tenicollis from slaughtered sheep and goats was carried out by creating Cysticercus species-specific and Cysticercus specific primers in Basrah governorate, southern Iraq. Study the molecular characterization of C. tenicollis and C. ovis from sheep and goats, by designing primer to Cysticercus spp. and specific primers to detect C. tenicollis and C. ovis. a total of 23 sheep (7 males, 16 females) and 23 goats (19 males, 4 females) were inspected. This investigation took place in Basrah province from November 2020 to November 2021, and the animal isolates were gathered throughout that time. The primers were developed as universal for the identification of Cysticercus species; they can amplify a portion of (ND1) and (COX1) genes of C. tenicollis and C. ovis, respectively. In general, Each cyst was identified as belonging to the genus T. hydatigenia by its unique mt-CO1 sequence, which yielded 267 bp product. This strain of T. hydatigenia is 100% identical to the Iraqi strain first isolated from Southern Iraq and entered into the Gene Bank under the designation "Iraqi strain," as shown by multiple sequence alignment of the nucleotide with previously published references (OK3556791, OK356792, OK356793, and OK356794).
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