Combination of gelatine -alginate hydrogel composite as a dual membrane for extra oral and Intraoral tissue repair.

Indugayathrie VT; Dr. Bargavi P

doi:10.69980/redvet.v25i1S.2383

Indugayathrie VT
Dr. Bargavi P

DOI: https://doi.org/10.69980/redvet.v25i1S.2383

Keywords: Gelatine, Alginate, dual membrane, tissue repair.

Abstract

Background: Tissue repair and regeneration are critical aspects of healthcare, particularly in the fields of maxillofacial surgery and dentistry. Hydrogel-based materials have emerged as promising candidates for tissue engineering due to their biocompatibility and tunable properties. The repair of extraoral and intraoral tissues poses unique challenges, as they are exposed to different physiological environments and have distinct functional requirements. Gelatine and alginate are widely used biomaterials known for their biocompatibility, bioactivity, and biodegradability. By combining these materials into a hydrogel composite, it is possible to develop a dual membrane that can be applied to various tissue repair scenarios.

Aim: The present study focused on the synthesis of Combination of gelatine -alginate hydrogel composite as a dual membrane for extra oral and Intraoral tissue repair.

Materials and Methods: Gelatine preparation: Gelatine is extracted from collagen-rich sources such as porcine or bovine skin through a controlled heating and enzymatic process.; Alginate preparation: Alginate is derived from brown algae and is typically obtained as a powder. Composite formation: The gelatine and alginate are mixed at appropriate ratios to form a homogenous hydrogel composite. The crosslinking mechanism can be achieved through ionic gelation or covalent crosslinking, depending on the desired properties. Characterization: The physical, mechanical, and biological properties of the gelatine-alginate hydrogel composite are evaluated using techniques such as rheology, scanning electron microscopy (SEM), and cell culture studies.

Results: In FUNCTIONAL GROUP: The functional groups present in the network are carboxylate group, amide, C=O, NH3; HAEMOLYSIS: Haemolysis percentage is less than 1% which indicates high bio compatibility.; CONTACT ANGLE: The hydrogel exhibited an average contact angle of 31.02°, indicating high surface hydrophilicity.; SEM ANALYSIS: Scanning electron microscopy revealed a uniform and interconnected porous network structure with pores ranging from 50–200 µm, supporting cell adhesion and nutrient diffusion.

Conclusion: The combination of gelatine and alginate in a hydrogel composite offers a promising dual membrane system for extraoral and intraoral tissue repair. The composite's biocompatibility, mechanical strength, and dual functionality make it a suitable candidate for a wide range of applications in maxillofacial surgery and dentistry. Further research and optimization are required to fully exploit the potential of this innovative hydrogel composite for tissue engineering and regenerative medicine.

Author Biographies

Indugayathrie VT

Saveetha Dental College and Hospitals Saveetha Institute of Medical and Technical Sciences Saveetha University, Chennai India

Dr. Bargavi P

Department of Oral Pathology Saveetha Dental College and Hospitals Saveetha Institute of Medical and Technical Sciences Saveetha University, Chennai India

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