Anti Microbial Assessment Of Chitosan Nanoparticle Incorporated With Gic Against Streptococcus Mutans And E Faecalis
Abstract
AIM : to study on anti microbial assessment of chitosan nanoparticle incorporated with gic against streptococcus mutans and e faecalis
MATERIALS AND METHOD: The given sample was collected and prepared by boiling it for 15 min. It was placed in the shaker for 3 days and Mueller Hinton agar was utilised for this activity to determine the zone of inhibition. The agar was sterilised for 15 mins at 121*C. Media was poured in the plates and left for solidification. The wells were cut using a 9mm sterile polystyrene tip and the test organisms were swabbed. The nanoparticles with different concentrations (25 µl,50 µl, 100 µl) were loaded and in the fourth well standard antibiotic amoxyrite was loaded. The plates were incubated for 24 hrs at 37*C. After incubation time the zone of inhibition was measured.
RESULT: The anti microbial activity assessed via chitosan nanoparticle incorporated with gic shows inhibition of bacterial growth,reduction in biofilm formation,synergistic effect with GIC,concentration depended response i.e, higher concentrations result in increased inhibition of bacterial growth or biofilm formation.
CONCLUSION: the antimicrobial assessment of chitosan nanoparticles incorporated with GIC against Streptococcus mutans and Enterococcus faecalis holds promise for improving dental material’s antimicrobial properties. However, further research and clinical trials are necessary to establish their efficacy, safety, and optimize their formulation for dental applications.
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