Preparation And Characterisation of Tcp Incorporated In Gelatin Scaffold
Keywords:
β-TCPGelatinChitosanTissue engineering scaffoldMesenchymal stem cell
Abstract
Aim- In this study, we suggest a new biocomposite scaffold composed of gelatin/α-TCP (tricalcium phosphate)/(GTS) which has enhanced mechanical strength and high level of cellular activity. Introduction- To fabricate gelatinscaffold, a temperature-controlled 3D printing process was used and appropriate printing conditions were selected based on rheological data and to show the feasibility as a biomedical scaffold for bone tissue regeneration. Materials and methods- The various physical and biological results, using MG63 (osteoblast-like cells), of the gelatin scaffold were compared with those of a pure gelatin (G) and gelatin/α-TCP (GT) composite scaffold. gelatin scaffolds showed enhanced mechanical properties in dry and wet state compared to those of the G and GT scaffolds. Also, significantly high cell-proliferation and differentiation of MG63 cells were observed in the gelatin scaffold. Results Therefore, the gelatin composite scaffold will be one of highly potential biomaterials to be used in bone regeneration.
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3. Alder KD, Lee I, Munger AM, Kwon H-K, Morris MT, Cahill SV, et al. Intracellular Staphylococcus aureus in bone and joint infections: A mechanism of disease recurrence, inflammation, and bone and cartilage destruction. Bone. 2020;141:115568. pmid:32745687
4. Mohamed W, Sommer U, Sethi S, Domann E, Thormann U, Schütz I, Lips KS, et al. Intracellular proliferation of S. aureus in osteoblasts and effects of rifampicin and gentamicin on S. aureus intracellular proliferation and survival. Eur Cells Mater. 2014;25(1473–2262 (Electronic)):258–68. pmid:25340805
5. Thompson K, Petkov S, Zeiter S, Sprecher CM, Richards RG, Moriarty TF, et al. Intraoperative loading of calcium phosphate-coated implants with gentamicin prevents experimental Staphylococcus aureus infection in vivo. PloS one. 2019;14(2):e0210402. pmid:3070769
6. Zou F, Jiang J, Lv F, Xia X, Ma X. Preparation of antibacterial and osteoconductive 3D-printed PLGA/Cu(I)@ZIF-8 nanocomposite scaffolds for infected bone repair. J Nanobiotechnology. 2020;18(1):39. pmid:32103765
7. Piarali S, Marlinghaus L, Viebahn R, Lewis H, Ryadnov MG, Groll J, et al. Activated Polyhydroxyalkanoate Meshes Prevent Bacterial Adhesion and Biofilm Development in Regenerative Medicine Applications. Front Bioeng Biotech. 2020;8:442. pmid:3267102
8. Munita JM, Arias CA, Kudva IT, Zhang Q. Mechanisms of Antibiotic Resistance. Microbiol Spectr. 2016;4(2):1–37. pmid:27227291
9. Rios-Arce ND, Schepper JD, Dagenais A, Schaefer L, Daly-Seiler CS, Gardinier JD, et al. Post-antibiotic gut dysbiosis-induced trabecular bone loss is dependent on lymphocytes. Bone. 2020;134:115269. pmid:3206167
10. Ledesma F, Ozcan B, Sun X, Medina SM, Landry MP. Nanomaterial Strategies for Delivery of Therapeutic Cargoes. Adv Funct Mater. 2021;32:2107174.
11. Su Y, Zhang B, Sun R, Liu W, Zhu Q, Zhang X, et al. PLGA-based biodegradable microspheres in drug delivery: recent advances in research and application. Drug Deliv. 2021;28(1):1397–418. pmid:3418494
12. Rizeq BR, Younes NN, Rasool K, Nasrallah GK. Synthesis, Bioapplications, and Toxicity Evaluation of Chitosan-Based Nanoparticles. Int J Mol Sci. 2019;20(22):5776. pmid:31744157
13. Eivazzadeh-Keihan R, Noruzi EB, Mehrban SF, Aliabadi HAM, Karimi M, Mohammadi A, et al. Review: the latest advances in biomedical applications of chitosan hydrogel as a powerful natural structure with eye-catching biological properties. J Mater Sci. 2022;57(6):3855–91.
14. Wang W, Meng Q, Li Q, Liu J, Zhou M, Jin Z, et al. Chitosan Derivatives and Their Application in Biomedicine. Int J Mol Sci. 2020;21(2):487. pmid:3194096317.
15. Jiménez-Gómez CP, Cecilia JA. Chitosan: A Natural Biopolymer with a Wide and Varied Range of Applications. Molecules. 2020;25(17):3981. pmid:3288289918.
16. Lin F, Wang Z, Xiang L, Deng L, Cui W. Charge‐Guided Micro/Nano‐Hydrogel Microsphere for Penetrating Cartilage Matrix. Adv Funct Mater. 2021;31:2107678. .
17. Fahimipour F, Rasoulianboroujeni M, Dashtimoghadam E, Khoshroo K, Tahriri M, Bastami F, et al. 3D printed TCP-based scaffold incorporating VEGF-loaded PLGA microspheres for craniofacial tissue engineering. Dent Mater. 2017;33(11):1205–16. pmid:288823620.
18. Yu X, Shen G, Shang Q, Zhang Z, Zhao W, Zhang P, et al. A Naringin-loaded gelatin-microsphere/nano-hydroxyapatite/silk fibroin composite scaffold promoted healing of critical-size vertebral defects in ovariectomised rat. Int J Biol Macromol. 2021;193:510–8. pmid:34710477
Published
2024-10-25
How to Cite
Aleena Alex, Dr. Subashree S, Sahana Scientist, & Dr. Karthikeyan G. (2024). Preparation And Characterisation of Tcp Incorporated In Gelatin Scaffold. Revista Electronica De Veterinaria, 25(2), 1927-1932. https://doi.org/10.69980/redvet.v25i2.2031
Section
Articles
