Changes in the activity of Metabolic and Antioxidant Enzymes in Fungicide treated Maize seedlings

  • Dr. Shobha.N
  • Dr. Savitha. G
Keywords: Metalaxyl, germination, Peroxidase (POX), Polyphenol Oxidase (PPO), Catalase (CAT), Phenyl alanine ammonia lyase (PAL) α-amylase, β-amylase, Protease

Abstract

The present investigation was aimed at finding out the enzymatic changes induced by the fungicide seed treatments in maize seedlings. Induction of various antioxidant enzymes and other defensive compounds is a common phenomenon in plants in response to different biotic and abiotic stresses.   Hydrolytic enzymes play a set of roles in the biochemical mechanism of germination. The present study gives an insight into a protective effect of the system with an increased production of proline and decreased protease activity, and hydrolytic enzymes. At the same time, increased defense enzymes were observed during the later stages of germination for a particular concentration of metalaxyl. This may be due to the synthesis of novel proteins involved in the defense mechanism and regulation process. This indicates the dual role of metalaxyl. Further work is in progress in understanding the structural and functional aspects of these enzymes.

Author Biographies

Dr. Shobha.N

Assistant Professor, Department of Biochemistry, Maharani’s Science College for Women, Mysuru, Karnataka, India

Dr. Savitha. G

Associate Professor, Department of Chemistry, Maharani’s Science College for Women, Mysuru, Karnataka, India

References

Akinlosottu and I.O.Akinyele, (1991). Effect of soaking, dehulling and fermentation on the oligosaccharides and nutrient content of cowpeas (Vigna unguiculatata). Foodchem. Vol.41, pp.43-53.

A.Kataria, B.M. Chanban and D.Punia, (1992). Digestibility of proteins and starch (in vitro) of ampidiploids (black gram x mung beang) as affected by domestic processing and cooking. Plant Foods Hum. Nutr. vol.42 (2), pp.117-125.

A.Negi.P.Boora and N.Khetapaul, (1996). Starch and Protein digestibility of newly released moth bean cultivars: Effect of soaking, dehulling, germination and pressure cooking. Nahrung, 329-331.

Abbaspour H. (2012). Effect of salt stress on lipid peroxidation, antioxidative enzymes, and proline accumulation in pistachio plants. J Med Plants Res. 6:526-529.

Aebi, H., (1984), Catalase in vitro, Method of Enzymology, 105:121-126.

Ahmed Adda, Zineb Regagba, Ahmed Latigui and Othmane Merah, (2014). Effect of Salt Stress on α-amylase Activity, Sugars Mobilization and Osmotic Potential of Phaseolus vulgaris L. Seeds Var. ‘Cocorose’ and ‘Djadida’ During Germination. Journal of Biological Sciences, 14: 370-375. DOI: 10.3923/jbs.2014.370.375.

Alfred M. Mayer, Eitan Harel (1979), Polyphenol oxidases in plants, Phytochemistry, Volume 18, Issue 2, Pages 193-215, ISSN 0031-9422, https://doi.org/10.1016/0031-9422(79)80057-6.

Averyanov A (2009). Oxidative burst and plant disease resistance. Front Biosci. (Elite Ed) 1: 142-152.

Baun, L.C., E.P. Palmiano, C.M. Perez, and B.O. Juliano (1970). Enzymes of starch metabolism in the developing rice grain. Plant physiol. 46: 429-434.

Bernfield, P. (1951). Enzymes of starch degradatuib abd stbtgesus, Advan Enzymol. 12:379-428.

Burda, S., W. Oleszek, and C.Y. Lee. (1990). Phenolic compounds and their changes in apples during maturation and cold storage. J. Agr. Food Chem. 38:945–948.

Chance, B. and Maehly, A.C. (1955) Assay of Catalase and Peroxidase. Methods in Enzymology,2,764-775. http://dx.doi.org/10.1016/S0076-6879(55)02300-8.

Dangl J, Jones J (2001). Plant pathogens and integrated defence responses to infection. Nature 411: 826-833.

Deshapande, A.A. and G.S. Swami: (1990). Gibberellin sensitivity in sorghum (Sorghum bicolor L. Moench) effect of methyl parathion. Ind. J. Exp.Biol., 28, 83-86.

Divekar, P. A., Narayana, S., Divekar, B. A., Kumar, R., Gadratagi, B. G., Ray, A., Singh, A. K., Rani, V., Singh, V., Singh, A. K., Kumar, A., Singh, R. P., Meena, R. S., & Behera, T. K. (2022). Plant Secondary Metabolites as Defense Tools against Herbivores for Sustainable Crop Protection. International Journal of Molecular Sciences, 23(5), 2690. https://doi.org/10.3390/ijms23052690

Doehlemann G, Ramon W, Robin J, Lars M, Bjo R, Fabien P, Mark S, Hnemann J, Uwe S, Regine K, Mper A (2008). Reprogramming a maize plant, transcriptional and metabolic changes induced by the fungal biotroph Ustilago maydis. The Plant J. 56: 181-195.

Essmann, J., Schmitz-Thom, I., Schon, H., Sonnewald, S., Weis, E. and Scharte, J. (2008). RNA interference-mediated repression of cell wall invertase impairs defense in source leaves of tobacco. Plant Physiol., 147: 1288-1299.

Evelyn P. Palmiano and Bienvenido O. Juliano, (1973). Changes in the activity of Some Hydolases, Peoxidase, and Catalase in the rice Seed during Germination. Plant Physiol, vol. 52, pp.274-277.

Fabiola Araceli Guzmán-Ortiz, Javier Castro-Rosas, Carlos Alberto Gómez- ldapa, Rosalva Mora-Escobedo, Adriana Rojas-León, María Luisa Rodríguez-Marín, Reyna Nallely Falfán-Cortés & Alma Delia Román-Gutiérrez (2019), Enzyme activity during germination of different cereals: A review, Food Reviews International, 35:3, 177-200, DOI: 10.1080/87559129.2018.1514623

Ferreira RB, Malo TS, Teixeira AN (1995) Catabolism of the seed storage proteins from Lupines albus: Fate of globulins during germination and seedling growth. Australian Journal of Plant Physiology. 22:373–381.

Fisher DJ, Hayes AL (1982) Mode of action of the systemic fungicides furalaxyl, metalaxyl and ofurace. Pestic. Sci. 13:330–339. DOI: 10.1002/ps.2780130316.

Fotopoulos vasileios (2005). Plant invertases: Review article structure, function and regulation of a diverse enzyme family. Journal of Biological Research, 4: 127-137.

Franklin Aragão Gondim, Enéas Gomes-Filho, José Hélio Costa, Nara Lídia Mendes Alencar, José Tarquinio Prisco (2012), Catalase plays a key role in salt stress acclimation induced by hydrogen peroxide pretreatment in maize, Plant Physiology and Biochemistry, Volume 56, Pages 62-71, ISSN 0981-9428, https://doi.org/10.1016/j.plaphy.2012.04.012.

Gepstin S, Han I, (1980). Evidence for the involvement of cytokinin in the regulation of proteolytic activity in cotyledons of germinating beans. Plant and Cell Physiology., 21(1):57-63.

Gracia, E., and F.M. Lajola,(1988). Starch transformation during banana ripening, the amylase and glucosidase behavior. J.Food.Science. 53, 1181-1186.

Gurpreet Singh and Daljit Kaur (2016) Studies on the Antioxidative stress responses of fungicides carbendazim and mancozeb in seedlings of brassica (Brassica compestris L.) International Research Journal Vol. 5(2), 57-62), E-ISSN 2319–1414.

Hammerschmidt, R., Nuckles, E.M. and Kuc, J. (1982). Association of enhanced peroxidase activity with induced systemic Resistance of cucumber to Colletotrichum lagenarium. Physiol. Plant Pathol. 20: 73-82.

Hao Z, Wang L, Huang F, Tao R (2012). Expression of defense genes and antioxidant defense responses in rice resistance to neck blast at the preliminary heading stage and full heading stage. Plant Physiol. Bioch. 57: 222-230.

Hussain, A., Tanveer, M., Brestic, M., Fatma, M., Iqbal, N., Hashem, A. (2020). Reactive oxygen species and abiotic stress tolerance in plants. Plant Cell Rep. 39 (3), 281–292.

ISTA. International rules for seed testing. In: Daper SR.(Ed), Rules (2003). International seed testing association, Zurich, Switzerland, 1-52.

Jablonkai, I. (2022). “Molecular defense mechanisms in plants to tolerate toxic action of heavy metal environmental pollution,” in Plant defense mechanisms, June (Intech Open). doi: 10.5772/intechopen.102330.

K.Muntz, (1996). Proteases and Proteolytic cleavage of storage proteins in developing and germinating dicotyledonous seeds. J.exp. Botany. Vol.47, pp.605-622.

Kunitz, M, (1947) Isolation of a crystalline protein compound of trypsin and of soybean trypsin- Inhibitor. J. Gen. Physiol., 30(4), 311-320.

Kusvuran, S., Kiran, S., & Ellialtioglu, S. S. (2016). Antioxidant Enzyme Activities and Abiotic Stress Tolerance Relationship in Vegetable Crops. InTech. doi: 10.5772/62235.

Kumar, N., Singh, H., Sharma, S. K. (2020). “Antioxidants: responses and importance in plant defense system,” in Sustainable agriculture in the era of climate change. Eds. Roychowdhury, R., Choudhury, S., Hasanuzzaman, M., Srivastava, S. (Cham: Springer), 251–264.

Leung, D.W.M. (2018). Studies of Catalase in Plants Under Abiotic Stress. In: Gupta, D., Palma, J., Corpas, F. (eds) Antioxidants and Antioxidant Enzymes in Higher Plants. Springer, Cham. https://doi.org/10.1007/978-3-319-75088-0_2)

Lowry, O.H., Rosenbrought, N.J., Farr, F.L. and Randall, R.J. (1951). Protein measurement with Folin phenol reagent.J. Biol. Chem. 193: 265-275.

Martinez M, Gómez-Cabellos S, Giménez MJ, Barro F, Diaz I and Diaz-Mendoza M (2019) Plant Proteases: From Key Enzymes in Germination to Allies for Fighting Human Gluten-Related Disorders. Front. Plant Sci. 10:721. doi: 10.3389/fpls.2019.00721

Massala, A., Legrand, M. and Fritig, B. (1980). Effectof aminooxyacetate, a competitive inhibitor ofphenylalanine ammonia lyase, on thehypersensitive resistance of tobacco to tobaccomosaic virus. Physiol. PI. Pathol. 16: 213-226.

Mhamdi, Amna & Queval, Guillaume & Chaouch, Sejir & Vanderauwera, Sandy & Van Breusegem, Frank & Noctor, Graham. (2010). Catalase function in plants: A focus on Arabidopsis mutants as stress-mimic models. Journal of experimental botany. 61. 4197-220. 10.1093/jxb/erq282.

Mohamed, Saleh & Al-Malki, Abdulrahman & Kumosani, Taha. (2009). Partial Purification and Characterization of Five α-amylases from a Wheat Local Variety (Balady) During Germination. Australian Journal of Basic and Applied Sciences. 3. 1740-1748.

Molina, A., Hunt, M. D. and Ryals, J. A. (1998). Impaired fungicide activity in plants blocked in disease resistance signal transduction. Plant Cell,10: 1903-1914.

Moloi Sellwane Jeanette, Ngara Rudo (2023), The roles of plant proteases and protease inhibitors in drought response: a review, Front. Plant Sci. Sec. Plant Abiotic Stress, Volume 14 –https://doi.org/10.3389/fpls.2023.1165845

Muntz K, Belozersky MA, Dunaevsky YE, Schlereth A, Tiedemann J. (2001) Stored proteinases and the initiation of storage protein mobilization in seeds during germination and seedling growth. Journal of Experimental Botany. 52:1741–1752.

Nijabat, A., Manzoor, S., Faiz, S., Naveed, N. H., Bolton, A., Khan, B. A., Ali, A., & Simon, P. (2023). Variation in Seed Germination and Amylase Activity of Diverse Carrot [Daucus carota (L.)] Germplasm under Simulated Drought Stress. HortScience, 58(2), 205-214. https://doi.org/10.21273/HORTSCI16806-22

Olsen OA (2001). ENDOSPERM DEVELOPMENT: Cellularization and Cell Fate Specification. Annu Rev Plant Physiol Plant Mol Biol. 52:233-267. doi: 10.1146/annurev.arplant.52.1.233. PMID: 11337398.

Prasad BN, Mathur SN (1983) Effect of metasystox and cumin-l on seed germination, reducing sugar content and amylase activity in Vigna mungo (L.) Hepper. Ind J Plant Physiol 2(24):209–213.

Rajeswari J, Ramakrishna Rao P. (2002), Storage protein degradation in germinating horse gram seeds. Indian J Plant Physiol. 7:314-320.

Sari, Afrima. (2021). The Effect of High Temperature on α-Amylase Enzyme Activity in the Germination of Several Rice Varieties (Oryza sativa L.). JERAMI Indonesian Journal of Crop Science. 3. 50-54. 10.25077/jijcs.3.2.50-54.2021.

Schlereth A, Standhardt D, Mock HP, Muntz K (2001). Stored cysteine proteinases start globulin breakdown in protein bodies of embryonic axis and cotyledons of germinating vetch (Vicia sativa L.) seeds. Planta, 212:718–727.

Shomali, A.; Das, S.; Arif, N.; Sarraf, M.; Zahra, N.; Yadav, V.; Aliniaeifard, S.; Chauhan, D.K.; Hasanuzzaman, M (2022) Diverse physiological roles of flavonoids in plant environmental stress responses and tolerance. Plants, 11, 3158.

Sturm, A. and Chrispeels, M. J, (1990). cDNA cloning of carrot extracellular b-fructosidase and its expression in response to wounding and bacterial infection. Plant cell, 2: 1107-1119.

Taneyama M, Okamoto T, Yamauchi D, Minamikawa T. (1996) Development of endopeptidase activity in cotyledons of Vigna mungo seedling: Effects of exogenously applied end products and plant hormones. Plant Cell and Physiology. 37:19–26.

Vieira de Silva J. (1969): In: Water and plant life: Problems and modern approaches (Eds.: O.L. Lange, L. Kappen and E.D. Schulze). Zpringer Verlag, New York.

Wang C, Tian Y, Wang X, Geng J, Jiang J, Yu H, Wang C (2010 Aug;). Lead-contaminated soil induced oxidative stress, defense response and its indicative biomarkers in roots of Vicia faba seedlings. Ecotoxicology. 19(6):1130-9.

Zhang J, Sun X. (2021) Recent advances in polyphenol oxidase-mediated plant stress responses. Phytochemistry. 181:112588. doi: 10.1016/j.phytochem.2020.112588. Epub 2020 Nov 21. PMID: 33232863.

Published
2022-07-28
How to Cite
Dr. Shobha.N, & Dr. Savitha. G. (2022). Changes in the activity of Metabolic and Antioxidant Enzymes in Fungicide treated Maize seedlings. Revista Electronica De Veterinaria, 23(4), 175-183. https://doi.org/10.69980/redvet.v23i4.1777
Section
Articles