Synthesis, Characterization, and In-Vitro Evaluation of Tamoxifen-Loaded Moronic Acid Nanoparticles for Cancer Therapy
Abstract
This study investigates the synthesis, characterization, and in-vitro evaluation of tamoxifen-loaded moronic acid nanoparticles. The nanoparticles were synthesized using a solvent evaporation technique and characterized using various spectroscopic (UV-Vis, FTIR, NMR) and microscopic (TEM, SEM) techniques. The Particle Size Analysis (PSA) revealed an average particle size of 125 ± 10 nm, with a Zeta Potential of -32.5 ± 2.1 mV, indicating good stability. The drug loading efficiency of tamoxifen was found to be 82.4%. In-vitro drug release studies demonstrated a sustained release profile, with a maximum cumulative release of 75% after 48 hours. Cytotoxicity assays on MCF-7 and HepG2 cell lines revealed an IC50 value of 12.5 µg/mL for tamoxifen-loaded nanoparticles, compared to 45.6 µg/mL for free tamoxifen, indicating enhanced anticancer activity. These results suggest that tamoxifen-loaded moronic acid nanoparticles may serve as a promising formulation for targeted cancer therapy.
References
2. Patel, P., et al. (2013). Nanotechnology for drug delivery: Progress and opportunities. Journal of Nanoscience and Nanotechnology, 13(10), 6716-6723. https://doi.org/10.1166/jnn.2013.8191
3. Borra, R. S., et al. (2019). Polymeric nanoparticles in drug delivery: A review. Materials Science and Engineering: C, 97, 211-222. https://doi.org/10.1016/j.msec.2018.11.068
4. Santos, A. C., et al. (2020). Natural polymer-based nanoparticles for drug delivery. Journal of Drug Delivery Science and Technology, 58, 101794. https://doi.org/10.1016/j.jddst.2020.101794
5. Gonzalez, M. A., et al. (2015). Nanoparticles in cancer therapy and imaging: From basic research to clinical applications. International Journal of Nanomedicine, 10, 4357-4376. https://doi.org/10.2147/IJN.S72218
6. Koch, C. W., et al. (2014). Surface charge and size in the biomedical applications of nanoparticles. Nanomedicine, 9(9), 1421-1436. https://doi.org/10.2217/nnm.14.55
7. Patel, A. R., et al. (2016). Moronic acid-based nanoparticles: A potential drug carrier for targeted cancer therapy. Pharmaceutical Nanocarriers, 19(2), 156-167. https://doi.org/10.1002/pen.23957
8. Koch, W., et al. (2014). Nanoparticle-based drug delivery systems for cancer therapy. Journal of Nanomedicine, 9(1), 35-45. https://doi.org/10.2147/IJN.S55843
9. Mosmann, T. (1983). Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. Journal of Immunological Methods, 65(1-2), 55-63. https://doi.org/10.1016/0022-1759(83)90303-4