PHB(Polyhydroxybutyrate) Production by Pseudomonas aeruginosa and its Application in Dye Decolorization
Abstract
Polyhydroxyalkanoates(PHAs) are class of biodegradable,biocompactible plastics composed of polyester of R-hydroxyalkanoic acids. Polyhydroxybutyrate(PHB) is a short chain of PHA that is naturally produced by several microorganisms as a reserve material. Its accumulated in bacteria as intracellular storage granules in the presence of excess carbon source and limited nitrogen source.
The present work focused on the extraction of PHB from a gram negative bacteria P.aeruginosa and its application in dye decolorization. It showed significant results in the degradation of crystal violet dye. After the 64 hours of incubation, a combination of PHB and Pseudomonas aeruginosa culture gives 92% color removal efficiency that is highest as compare to individual treatment of PHB and Pseudomonas aeruginosa alone with dye.While individually treated dye with PHB and Pseudomonas aeruginosa alone gives 68% and 79.50% respectively. Thus, the combination of PHB and Pseudomonas aeruginosa culture is found to be significantly effective in decolorization of dye.
References
2. Charles David, M. Arivazhagan, M. N. Balamurali, and Dhivya Shanmugarajan (2015) . Bipoymer Research International, http://dx.doi.org/10.1155/2015/195879.
3. Rawia F. Gamal, Hemmat M. Abdelhady, Taha A. Khodair, Tarek S. El-Tayeb, Enas A. Hassan, Khadiga A. Aboutaleb (2013) Semi-scale production of PHAs from waste frying oil by Pseudomonas fluorescens S48, Brazilian journal of Micobiology. 44, 2, 539-549.
4. Blaithín McAdam, Margaret Brennan Fournet, Paul McDonald and Marija Mojicevic (2020). Production of Polyhydroxybutyrate (PHB) and Factors Impacting Its Chemical and Mechanical Characteristics, Polymers 12(12):2908. doi: 10.3390/polym12122908.
5. Nehra K, Chhabra N, Sidhu P, Lathwal P, Rana J S (2017). Molecular identification and characterization of Poly-β-hydroxybutyrate (PHB) producing bacteria isolated from contaminated soils. Asian Journal of Microbiology, Biotechnology and Environmental Sciences , 17:281-290.
6. Pachekoski WM, Agnelli JAM, Belem LP (2009)Thermal, mechanical and morphological properties of poly (hydroxybutyrate) and polypropylene blends after processing. Materials Research , 12(2):159-164.
7. Rydz J, Wanda S, Mariya K, and Darinka C (2015). Polyester-Based (Bio) Degradable Polymers as Environmentally Friendly Materials for Sustainable Development. International Journal of Molecular Sciences 16(1): 564–596.
8. Singh G, Mittal A, Kumari A, Goel A, Aggarwal NK, Yadav A(2011) Optimization of Poly-B-Hydroxybutyrate Production from Bacillus species. European Journal of Biological Sciences 3 (4): 112-116.
9. Ranganadha Reddy Aluru,(2020). Screening and Biochemical Characterization of PHB Producing Bacterium Isolated from Costal Region of Andhra Pradesh, Environmental and Earth Sciences Research Journal 7 (3),116-120.
10. Devaraj Sabarinathan, Sabapathy Poorna Chandrika, Pitchaikannu Venkatraman, Murugesh Easwaran, Chandrasekaran Senbagavadivoo Sureka, Kathirvel Preethi. (2018). Informatics in Medicine Unlocked. (11), 61-67.
11. Anteneh Getachew, Fantahun Woldesenbet. (2016) Production of biodegradable plastic by polyhydroxy butyrate (PHB) accumulating bacteria using low cost agricultural waste material, BMC Research notes. 9:509.
12. Reddy, A.R, Prabhakar, K.V., Venkateswarulu, T.C., Krupanidhi, S., Nazneen Bobby, Md., Abraham Peele, K., Sudhakar, P., Vijetha, P. (2020). Statistical optimization of PolyHydroxy Butyrate (PHB) production characterisation, substrate extraction and process modelling for the production of polyhydroxybutyrate by Cupriavidus necator. Bioresource Technology, 222: 242- 251.
