Influence of Spermatozoid Qualitative Indicators on Cow Fertility when Applying Artificial Insemination and Oocyte Fertility under in Vitro Conditions

  • Vladimir Aleksandrovich Pleshkov, Tatyana Vladimirovna Zubova, Oksana Vladimirovna Smolovskaya, Anna Vyacheslavovna Semechkova, Roman Olegovich Kasyanov, Filipp Nikolaevich Khitriy

Abstract

The aim of the research was to study the influence of the sperm cells’ qualitative parameters on cow fertility at artificial insemination and fertilization of oocytes under in vitro conditions. The research methodology provides two independent experiments: the first one is to assess the influence of sperm qualitative indicators on the fertility of cows when applying artificial insemination; the second one is to assess the influence of qualitative indicators of sperm production during fertilization of oocytes in vitro. Studies were carried out on the qualitative parameters of sperm (sperm activity, number of live and dead sperm, acrosome integrity) from 6 different breeding bulls, as well as the effect of these indicators on the fertility of cows in the estruation stage and the fertility of oocytes in vitro. In the course of the first experiment, it was found that the bulls’ sperm samples had an activity of 30.0±2.72% - 40.0±2.71%; indicators of live and dead sperm were 0.8-1%; the acrosome integrity corresponded to the norm (from 15.7±1.5 to 23.7±3.9%). Qualitative sperm indicators of the bull named Bokar-M (sperm activity (40.0±2.71%), a low number of sperm cells with a damaged acrosome (15.7±1.5%)) positively influenced the fertility of cows inseminated at the first menstruation (50%), their service period was 92.5±1.8 days. In the course of the second experiment, it was found that sperm samples from bulls had an activity of 33.0±2.3% - 42.0±2.10%, indicators of live and dead sperm when stained with eosin from 0.8-1%; the integrity of the sperm acrosome corresponded to the norm and was on average marked at the level from 25.2±1.8 to 36.3±2.2%. Better results at in vitro fertilization of oocytes (50% of fertilization) were obtained using the semen of the bull named Matthias-4593.

Published
2022-03-24
How to Cite
Anna Vyacheslavovna Semechkova, Roman Olegovich Kasyanov, Filipp Nikolaevich Khitriy, V. A. P. T. V. Z. O. V. S. (2022). Influence of Spermatozoid Qualitative Indicators on Cow Fertility when Applying Artificial Insemination and Oocyte Fertility under in Vitro Conditions. Revista Electronica De Veterinaria, 14 - 23. Retrieved from https://www.veterinaria.org/index.php/REDVET/article/view/157
Section
Articles