Eucalyptus Plant Extract is a Novel Agent for Disrupting Bacterial Biofilms and Inhibiting Microbial Growth
Keywords:
Eucalyptus Plant Extract, biofilm, Gram-Positive Bacteria, Minimum inhibitory concentration (MIC),, gas chromatography-mass spectrometry (GC/MS)
Abstract
Plant-derived chemicals with biological capabilities have become a viable option for microbial control in recent years. The antimicrobial effects of essential oils extracted from dry Eucalyptus staggering leaves have not been described, despite the antibacterial capabilities of Greens-derived essential oils from eucalyptus sp leaves being been established. The study purposed the eucalyptus plant extract for disrupting bacterial biofilms and inhibiting microbial growth of E. staigeriana (EOdlES) and determining their chemical composition. Analyze the antibacterial and antibiofilm effects of EOdlES on gram-positive and negative bacteria invitro. Enteric bacteria faecal, the impermeable along with multi-resistant microbe obtained from dietary and clinical specimens. We utilized the gas chromatography-mass spectrometry (GC/MS) for EOdlES characterization. There were a total of 26 bacterial strains used in this research; there were 11 employed as the standard, while the remaining 15 were antibiotic- and multidrug-resistant E. faecalis. The disc diffusion technique was used to test the antimicrobial efficacy of EOdlES against gram-positive and gram-negative bacteria. The microbiota diffusion method determined the minimum inhibitory concentration (MIC) value. Microtiter plate analysis was used to measure the antibiofilm effects. Twenty-one chemicals were isolated with oxygenated monoterpenes being the largest chemical family. Only gram-positive bacteria were killed by EOdlES antibacterial properties. The MIC is lowest for the regard E. faecal is strain followed by the resistant and multiresistant strains. Although EOdlES may prevent bio films from forming, it has little effect on existing bio films. This study indicates that EOdlES is an effective strategy for lowering the frequency of dangerous gram-positive resistant bacteria within medical and food industrial environments.
References
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[5] Nuță, D.C., Limban, C., Chiriță, C., Chifiriuc, M.C., Costea, T., Ioniță, P., Nicolau, I. and Zarafu, I., 2021. Contribution of essential oils to the fight against microbial biofilms—A review. Processes, 9(3), p.537.
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[12] Vazquez, N.M., Moreno, S. and Galván, E.M., 2022. Exposure of multidrug-resistant Klebsiella pneumoniae biofilms to 1, 8-cineole leads to bacterial cell death and biomass disruption. Biofilm, 4, p.100085.
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[14] Mohammadi, M., Masoumipour, F., Hassanshahian, M. and Jafarinasab, T., 2019. Study the antibacterial and antibiofilm activity of Carum copticum against antibiotic-resistant bacteria in planktonic and biofilm forms. Microbial Pathogenesis, 129, pp.99-105.
[15] Al-Qaysi, A.M.K., Al-Ouqaili, M.T. and Al-Meani, S.A., 2020. Ciprofloxacin-and gentamicin-mediated inhibition of Pseudomonas aeruginosa biofilms is enhanced when combined with the volatile oil from Eucalyptus camaldulensis. Syst Rev Pharm, 11, pp.98-105.
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[29] Bogdanov, M., Pyrshev, K., Yesylevskyy, S., Ryabichko, S., Boiko, V., Ivanchenko, P., Kiyamova, R., Guan, Z., Ramseyer, C. and Dowhan, W., 2020. Phospholipid distribution in the cytoplasmic membrane of Gram-negative bacteria is highly asymmetric, dynamic, and cell shape-dependent. Science Advances, 6(23), p.eaaz6333.
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Published
2023-03-10
How to Cite
Roopashree, Pandey, S. H., & Chandra, P. (2023). Eucalyptus Plant Extract is a Novel Agent for Disrupting Bacterial Biofilms and Inhibiting Microbial Growth. Revista Electronica De Veterinaria, 24(1), 101 - 115. Retrieved from https://www.veterinaria.org/index.php/REDVET/article/view/369
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